The effect of cholesterol on the structure of phosphatidylcholine bilayers
- PMID: 718889
- DOI: 10.1016/0005-2736(78)90110-4
The effect of cholesterol on the structure of phosphatidylcholine bilayers
Abstract
The effect of cholesterol on the structure of phosphatidylcholine bilayer was investigated by X-ray diffraction methods. Electron density profiles at 5 A resolution along with chain tilt and chain packing parameters were obtained and compared for phosphatidylcholine/cholesterol bilayers and for pure phosphatidylcholine bilayers in both the gel and liquid crystalline states. The cholesterol in the bilayers was localized by noting the position of discrete elevations in the electron density profiles. Cholesterol can either increase or decrease the width of the bilayer depending on the physical state and chain length of the lipid before the introduction of cholesterol. For saturated phosphatidylcholines containing 12--16 carbons per chain, cholesterol increases the width of the bilayer as it removes the chain tilt from gel state lipids or increases the trans conformations of the chains for liquid crystalline lipids. However, cholesterol reduces the width of 18 carbon chain bilayers below the phase transition temperature as the long phospholipid chains must deform or kink to accomodate the significantly shorter cholesterol molecule. Although cholesterol has a marked effect on hydrocarbon chain organization, it was found that, within the resolution limits of the data, the phosphatidylcholine head group conformation is unchanged by the addition of cholesterol to the bilayer. The head group is oriented parallel to the plane of the bilayer for phosphatidylcholine in the gel and liquid crystalline states and this orientation is not changed by the addition of cholesterol.
Similar articles
-
New structural model for mixed-chain phosphatidylcholine bilayers.Biochemistry. 1984 Aug 28;23(18):4038-44. doi: 10.1021/bi00313a005. Biochemistry. 1984. PMID: 6487589
-
A DSC and FTIR spectroscopic study of the effects of the epimeric 4-cholesten-3-ols and 4-cholesten-3-one on the thermotropic phase behaviour and organization of dipalmitoylphosphatidylcholine bilayer membranes: comparison with their 5-cholesten analogues.Chem Phys Lipids. 2014 Jan;177:71-90. doi: 10.1016/j.chemphyslip.2013.11.008. Epub 2013 Dec 1. Chem Phys Lipids. 2014. PMID: 24296232
-
Effects of lysophosphatidylcholines on phosphatidylcholine and phosphatidylcholine/cholesterol liposome systems as revealed by 31P-NMR, electron microscopy and permeability studies.Biochim Biophys Acta. 1981 Dec 7;649(2):211-20. doi: 10.1016/0005-2736(81)90408-9. Biochim Biophys Acta. 1981. PMID: 7317392
-
Cholesterol packing, crystallization and exchange properties in phosphatidylcholine vesicle systems.Hepatology. 1990 Sep;12(3 Pt 2):75S-80S; discussion 80S-82S. Hepatology. 1990. PMID: 2210662 Review.
-
Mixed-chain phospholipids and interdigitated bilayer systems.Klin Wochenschr. 1990 Feb 1;68(3):149-65. doi: 10.1007/BF01649079. Klin Wochenschr. 1990. PMID: 2179621 Review.
Cited by
-
Nonuniversal impact of cholesterol on membranes mobility, curvature sensing and elasticity.Nat Commun. 2023 Dec 11;14(1):8038. doi: 10.1038/s41467-023-43892-x. Nat Commun. 2023. PMID: 38081812 Free PMC article.
-
Role of long non-coding RNAs in cancer: From subcellular localization to nanoparticle-mediated targeted regulation.Mol Ther Nucleic Acids. 2023 Jul 16;33:774-793. doi: 10.1016/j.omtn.2023.07.009. eCollection 2023 Sep 12. Mol Ther Nucleic Acids. 2023. PMID: 37655045 Free PMC article. Review.
-
Polymorphism, Nanostructures, and Barrier Properties of Ceramide-Based Lipid Films.ACS Omega. 2022 Dec 23;8(1):422-435. doi: 10.1021/acsomega.2c04924. eCollection 2023 Jan 10. ACS Omega. 2022. PMID: 36643519 Free PMC article.
-
Formulation of tizanidine hydrochloride-loaded provesicular system for improved oral delivery and therapeutic activity employing a 23 full factorial design.Drug Deliv Transl Res. 2023 Feb;13(2):580-592. doi: 10.1007/s13346-022-01217-3. Epub 2022 Aug 4. Drug Deliv Transl Res. 2023. PMID: 35927549 Free PMC article.
-
Nanosecond Pulsed Electric Field (nsPEF): Opening the Biotechnological Pandora's Box.Int J Mol Sci. 2022 May 31;23(11):6158. doi: 10.3390/ijms23116158. Int J Mol Sci. 2022. PMID: 35682837 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources