Evidence against either a premature stop codon or the absence of obese gene mRNA in human obesity
- PMID: 7769141
- PMCID: PMC295988
- DOI: 10.1172/JCI118007
Evidence against either a premature stop codon or the absence of obese gene mRNA in human obesity
Abstract
Obese (ob) gene expression in abdominal subcutaneous adipocytes from lean and obese humans was examined. The full coding region of the ob gene was isolated from a human adipocyte cDNA library. Translation of the insert confirmed the reported amino acid sequence. There was no difference in the sequence of an reverse transcription PCR product of the coding region from five lean and five obese subjects. The nonsense mutation in the ob mouse which results in the conversion of arginine 105 to a stop codon was not present in human obesity. In all 10 human cDNAs, arginine 105 was encoded by CGG, consequently two nucleotide substitutions would be required to result in a stop codon. To compare the amount of ob gene expression in lean and obese individuals, radiolabed primer was used in the PCR reaction with beta-actin as a control. There was 72% more ob gene expression (P < 0.01) in eight obese subjects (body mass index, BMI = 42.8 +/- 2.7) compared to eight lean controls (BMI = 22.4 +/- 0.8). Regression analysis indicated a positive correlation between BMI and the amount of ob message (P < 0.005). There was no difference in the amount of beta-actin expression in the two groups. These results provide evidence that ob gene expression is increased in human obesity; furthermore, the mutations present in the mouse ob gene were not detected in the human mRNA population.
Comment in
-
Neutrophil chemoattractants generated in two phases during reperfusion of ischemic myocardium in the rabbit. Evidence for a role for C5a and interleukin-8.J Clin Invest. 1995 Jun;95(6):2720-8. doi: 10.1172/JCI117974. J Clin Invest. 1995. PMID: 7769111 Free PMC article.
Similar articles
-
Molecular cloning, sequencing and expression of obese gene in the Chinese.Chin Med J (Engl). 2000 Apr;113(4):350-3. Chin Med J (Engl). 2000. PMID: 11775234
-
Molecular cloning of rat obese cDNA and augmented gene expression in genetically obese Zucker fatty (fa/fa) rats.J Clin Invest. 1995 Sep;96(3):1647-52. doi: 10.1172/JCI118204. J Clin Invest. 1995. PMID: 7657834 Free PMC article.
-
Human obese gene expression. Adipocyte-specific expression and regional differences in the adipose tissue.Diabetes. 1995 Jul;44(7):855-8. doi: 10.2337/diab.44.7.855. Diabetes. 1995. PMID: 7789654
-
Multihormonal control of ob gene expression and leptin secretion from cultured human visceral adipose tissue: increased responsiveness to glucocorticoids in obesity.J Clin Endocrinol Metab. 1998 Mar;83(3):902-10. doi: 10.1210/jcem.83.3.4644. J Clin Endocrinol Metab. 1998. PMID: 9506746 Review.
-
[ob protein--product of expressing an obesity gene and some aspects of modern-day endocrinology].Biokhimiia. 1996 Jun;61(6):984-92. Biokhimiia. 1996. PMID: 9011250 Review. Russian.
Cited by
-
Aging and Adiposity-Focus on Biological Females at Midlife and Beyond.Int J Mol Sci. 2024 Mar 4;25(5):2972. doi: 10.3390/ijms25052972. Int J Mol Sci. 2024. PMID: 38474226 Free PMC article. Review.
-
Hypothalamic-Ovarian axis and Adiposity Relationship in Polycystic Ovary Syndrome: Physiopathology and Therapeutic Options for the Management of Metabolic and Inflammatory Aspects.Curr Obes Rep. 2024 Mar;13(1):51-70. doi: 10.1007/s13679-023-00531-2. Epub 2024 Jan 3. Curr Obes Rep. 2024. PMID: 38172476 Free PMC article. Review.
-
Identification of a GABAergic neural circuit governing leptin signaling deficiency-induced obesity.Elife. 2023 Apr 12;12:e82649. doi: 10.7554/eLife.82649. Elife. 2023. PMID: 37043384 Free PMC article.
-
Serum 25OHD3 of Obese Mice Is Affected by Liver Injury and Correlates with Testosterone Levels and Sperm Motility.Obes Facts. 2021;14(5):559-567. doi: 10.1159/000518199. Epub 2021 Aug 20. Obes Facts. 2021. PMID: 34515192 Free PMC article.
-
From leptin to lasers: the past and present of mouse models of obesity.Expert Opin Drug Discov. 2021 Jul;16(7):777-790. doi: 10.1080/17460441.2021.1877654. Epub 2021 Jan 29. Expert Opin Drug Discov. 2021. PMID: 33472452 Free PMC article.
References
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Miscellaneous