A heterodimeric coiled-coil protein required for mitotic chromosome condensation in vitro
- PMID: 7954811
- DOI: 10.1016/0092-8674(94)90254-2
A heterodimeric coiled-coil protein required for mitotic chromosome condensation in vitro
Abstract
We report here a chromosomal protein that plays an essential role in mitotic chromosome condensation in Xenopus egg extracts. Two polypeptides, designated XCAP-C and XCAP-E, were found to associate with each other in the extracts, presumably forming a heterodimer. During chromosome assembly in mitotic extracts, XCAP-C/E was recruited to the chromatin and formed a discrete internal structure within assembled chromosomes. Antibody blocking experiments showed that XCAP-C function is required for both assembly and structural maintenance of mitotic chromosomes in vitro. Deduced amino acid sequences revealed that the two polypeptides share common structural motifs, consisting of an N-terminal NTP-binding domain, two central coiled-coil regions, and a C-terminal conserved domain. These motifs are highly conserved in a protein family, members of which have been identified recently in both prokaryotes and eukaryotes.
Similar articles
-
A human condensin complex containing hCAP-C-hCAP-E and CNAP1, a homolog of Xenopus XCAP-D2, colocalizes with phosphorylated histone H3 during the early stage of mitotic chromosome condensation.Mol Cell Biol. 2000 Sep;20(18):6996-7006. doi: 10.1128/MCB.20.18.6996-7006.2000. Mol Cell Biol. 2000. PMID: 10958694 Free PMC article.
-
Condensins, chromosome condensation protein complexes containing XCAP-C, XCAP-E and a Xenopus homolog of the Drosophila Barren protein.Cell. 1997 May 16;89(4):511-21. doi: 10.1016/s0092-8674(00)80233-0. Cell. 1997. PMID: 9160743
-
Identification of two distinct human SMC protein complexes involved in mitotic chromosome dynamics.Proc Natl Acad Sci U S A. 1998 Oct 27;95(22):12906-11. doi: 10.1073/pnas.95.22.12906. Proc Natl Acad Sci U S A. 1998. PMID: 9789013 Free PMC article.
-
Shaping the metaphase chromosome: coordination of cohesion and condensation.Bioessays. 2001 Oct;23(10):924-35. doi: 10.1002/bies.1133. Bioessays. 2001. PMID: 11598959 Review.
-
The SMC proteins and the coming of age of the chromosome scaffold hypothesis.Bioessays. 1995 Sep;17(9):759-66. doi: 10.1002/bies.950170905. Bioessays. 1995. PMID: 8763828 Review.
Cited by
-
Rules of engagement for condensins and cohesins guide mitotic chromosome formation.bioRxiv [Preprint]. 2024 Apr 30:2024.04.18.590027. doi: 10.1101/2024.04.18.590027. bioRxiv. 2024. PMID: 38659940 Free PMC article. Preprint.
-
Recombinant cyclin B-Cdk1-Suc1 capable of multi-site mitotic phosphorylation in vitro.PLoS One. 2024 Mar 25;19(3):e0299003. doi: 10.1371/journal.pone.0299003. eCollection 2024. PLoS One. 2024. PMID: 38527022 Free PMC article.
-
Single-Molecule Approaches to Study DNA Condensation.Methods Mol Biol. 2024;2740:1-19. doi: 10.1007/978-1-0716-3557-5_1. Methods Mol Biol. 2024. PMID: 38393466
-
Regulation of condensin II by self-suppression and release mechanisms.Mol Biol Cell. 2024 Feb 1;35(2):ar21. doi: 10.1091/mbc.E23-10-0392. Epub 2023 Dec 13. Mol Biol Cell. 2024. PMID: 38088875 Free PMC article.
-
The material properties of mitotic chromosomes.Curr Opin Struct Biol. 2023 Aug;81:102617. doi: 10.1016/j.sbi.2023.102617. Epub 2023 Jun 6. Curr Opin Struct Biol. 2023. PMID: 37279615 Free PMC article. Review.
Publication types
MeSH terms
Substances
Associated data
- Actions
- Actions
LinkOut - more resources
Full Text Sources
Other Literature Sources
