[Construction of the genomic library for cyanobacteria Synechocystis sp. PCC 6803 using a cosmid]
[Article in
Russian]
- PMID: 8045394
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[Construction of the genomic library for cyanobacteria Synechocystis sp. PCC 6803 using a cosmid]
[Article in
Russian]
Genetika.
1994 Apr.
Abstract
The gene library of the phototrophic cyanobacterium Synechocystis PCC6803 was constructed in the cosmid vector Lorist6. About -3 x 10(3) recombinant clones were obtained, of which 760 were selected for further work. Each clone was kept in an individual well of a microtiter plate. The clone grids obtained were hybridized with two DNA probes, which contained the ndhE and dfr genes of Synechocystis PCC6803. Two groups of probe-overlapping cosmids (11 and 8 clones respectively), containing insertions averaging of 39 kb, were isolated. The capacity of the gene library is nine equivalents of the Synechocystis PCC6803 genome and its representativity exceeds 99%.
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