Characterization of human immunodeficiency virus type 1 gp120 binding to liposomes containing galactosylceramide
- PMID: 8057468
- PMCID: PMC236994
- DOI: 10.1128/JVI.68.9.5890-5898.1994
Characterization of human immunodeficiency virus type 1 gp120 binding to liposomes containing galactosylceramide
Abstract
Human immunodeficiency virus type 1 (HIV-1) infects some cell types which lack CD4, demonstrating that one or more alternative viral receptors exist. One such receptor is galactosylceramide (GalCer), a glycosphingolipid distributed widely in the nervous system and in colonic epithelial cells. Using a liposome flotation assay, we found that the HIV-1 surface glycoprotein, gp120, quantitatively bound to liposomes containing GalCer but not to liposomes containing phospholipids and cholesterol alone. Binding was saturable and was inhibited by preincubating liposomes with anti-GalCer antibodies. We observed less efficient binding of gp120 to liposomes containing lactosylceramide, glucosylceramide, and galactosylsulfate, whereas no binding to liposomes containing mixed gangliosides, psychosine, or sphingomyelin was detected. Binding to GalCer was rapid, largely independent of temperature and pH, and stable to conditions which remove most peripheral membrane proteins. By contrast, gp120 bound to lactosylceramide could be removed by 2 M potassium chloride or 3 M potassium thiocyanate, demonstrating a less stable interaction. Removal of N-linked oligosaccharides on gp120 did not affect binding efficiency. However, as previously observed for CD4 binding, heat denaturation of gp120 prevented binding to GalCer. Finally, binding was critically dependent on the concentration of GalCer in the target membrane, suggesting that binding to glycolipid-rich domains occurs and that GalCer conformation may be important for gp120 recognition.
Similar articles
-
Suramin inhibits binding of the V3 region of HIV-1 envelope glycoprotein gp120 to galactosylceramide, the receptor for HIV-1 gp120 on human colon epithelial cells.J Biol Chem. 1994 Sep 30;269(39):24349-53. J Biol Chem. 1994. PMID: 7929093
-
Binding of human immunodeficiency virus type I (HIV-1) gp120 to galactosylceramide (GalCer): relationship to the V3 loop.Virology. 1994 Jun;201(2):206-14. doi: 10.1006/viro.1994.1287. Virology. 1994. PMID: 8184533
-
Galactosyl ceramide or a derivative is an essential component of the neural receptor for human immunodeficiency virus type 1 envelope glycoprotein gp120.Proc Natl Acad Sci U S A. 1991 Aug 15;88(16):7131-4. doi: 10.1073/pnas.88.16.7131. Proc Natl Acad Sci U S A. 1991. PMID: 1871126 Free PMC article.
-
[Structure and function of oligosaccharide chains of the envelope glycoprotein gp120 of human immunodeficiency virus type 1].Nihon Rinsho. 1992 Jun;50(6):1419-29. Nihon Rinsho. 1992. PMID: 1518160 Review. Japanese.
-
CD4 activation of HIV fusion.Int J Cell Cloning. 1992 Nov;10(6):323-32. doi: 10.1002/stem.5530100603. Int J Cell Cloning. 1992. PMID: 1281202 Review.
Cited by
-
The Chemical Inhibitors of Endocytosis: From Mechanisms to Potential Clinical Applications.Cells. 2023 Sep 19;12(18):2312. doi: 10.3390/cells12182312. Cells. 2023. PMID: 37759535 Free PMC article. Review.
-
HIV and SIV Envelope Glycoproteins Interact with Glycolipids and Lipids.Int J Mol Sci. 2023 Jul 21;24(14):11730. doi: 10.3390/ijms241411730. Int J Mol Sci. 2023. PMID: 37511488 Free PMC article.
-
HIV-1-Specific IgA Monoclonal Antibodies from an HIV-1 Vaccinee Mediate Galactosylceramide Blocking and Phagocytosis.J Virol. 2018 Mar 14;92(7):e01552-17. doi: 10.1128/JVI.01552-17. Print 2018 Apr 1. J Virol. 2018. PMID: 29321320 Free PMC article.
-
Vaccine-induced HIV-1 envelope gp120 constant region 1-specific antibodies expose a CD4-inducible epitope and block the interaction of HIV-1 gp140 with galactosylceramide.J Virol. 2014 Aug;88(16):9406-17. doi: 10.1128/JVI.01031-14. Epub 2014 Jun 11. J Virol. 2014. PMID: 24920809 Free PMC article.
-
Human immunodeficiency virus gp120-induced apoptosis of human neuroblastoma cells in the absence of CXCR4 internalization.J Neurovirol. 2006 Jun;12(3):211-8. doi: 10.1080/13550280600848373. J Neurovirol. 2006. PMID: 16877302 Free PMC article.
References
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials