Molecular cloning and characterization of dTAFII30 alpha and dTAFII30 beta: two small subunits of Drosophila TFIID
- PMID: 8276241
- DOI: 10.1101/gad.7.12b.2587
Molecular cloning and characterization of dTAFII30 alpha and dTAFII30 beta: two small subunits of Drosophila TFIID
Abstract
The multisubunit transcription factor TFIID is an essential component of the RNA polymerase II initiation apparatus. Recent studies suggest that TFIID subunits, or TAFs associated with the TATA-binding protein (TBP), play a critical role in modulating transcriptional activation by sequence-specific DNA-binding factors. Thus far, six of the largest TAFs associated with Drosophila TFIID have been cloned and partially characterized. Here, we report the molecular cloning, expression, and subunit interaction specificities of two small molecular mass TAFs. Both dTAFII30 alpha and dTAFII30 beta are associated with TFIID via interactions with other TAFs, including dTAFII250, dTAFII150, and dTAFII110. In addition, dTAFII30 alpha also contacts dTBP. The carboxy-terminal half of dTAFII110 was found to contact a short 67-amino-acid region of dTAFII30 alpha, which is predicted to form two potential alpha-helices, one of which is amphipathic. Interestingly, dTAFII30 alpha also appears to multimerize through its carboxy-terminal region. Although neither dTAFII30 alpha nor dTAFII30 beta have been found to interact with specific activators thus far, it is intriguing that both bind other TAFs such as dTAFII110 and dTAFII150, which are the targets of activation domains. Our studies suggest that both of the small subunits of TFIID play a role in the assembly of the complex and may contribute to the stability of multiple TAF-TAF interactions.
Similar articles
-
The dTAFII80 subunit of Drosophila TFIID contains beta-transducin repeats.Nature. 1993 May 13;363(6425):176-9. doi: 10.1038/363176a0. Nature. 1993. PMID: 8483503
-
Drosophila TAFII150: similarity to yeast gene TSM-1 and specific binding to core promoter DNA.Science. 1994 May 13;264(5161):933-41. doi: 10.1126/science.8178153. Science. 1994. PMID: 8178153
-
Drosophila TAFII40 interacts with both a VP16 activation domain and the basal transcription factor TFIIB.Cell. 1993 Nov 5;75(3):519-30. doi: 10.1016/0092-8674(93)90386-5. Cell. 1993. PMID: 8221891
-
TAFs mediate transcriptional activation and promoter selectivity.Trends Biochem Sci. 1996 Sep;21(9):338-42. Trends Biochem Sci. 1996. PMID: 8870497 Review.
-
Mechanisms of transcriptional activation and repression can both involve TFIID.Philos Trans R Soc Lond B Biol Sci. 1996 Apr 29;351(1339):517-26. doi: 10.1098/rstb.1996.0050. Philos Trans R Soc Lond B Biol Sci. 1996. PMID: 8735274 Review.
Cited by
-
Proteomics reveals a physical and functional link between hepatocyte nuclear factor 4alpha and transcription factor IID.J Biol Chem. 2009 Nov 20;284(47):32405-12. doi: 10.1074/jbc.M109.017954. Epub 2009 Oct 5. J Biol Chem. 2009. PMID: 19805548 Free PMC article.
-
Yeast two-hybrid map of Arabidopsis TFIID.Plant Mol Biol. 2007 May;64(1-2):73-87. doi: 10.1007/s11103-007-9135-1. Epub 2007 Mar 6. Plant Mol Biol. 2007. PMID: 17340043
-
The coactivator dTAF(II)110/hTAF(II)135 is sufficient to recruit a polymerase complex and activate basal transcription mediated by CREB.Proc Natl Acad Sci U S A. 2001 Nov 6;98(23):13078-83. doi: 10.1073/pnas.241337698. Epub 2001 Oct 30. Proc Natl Acad Sci U S A. 2001. PMID: 11687654 Free PMC article.
-
Human Taf(II)130 is a coactivator for NFATp.Mol Cell Biol. 2001 May;21(10):3503-13. doi: 10.1128/MCB.21.10.3503-3513.2001. Mol Cell Biol. 2001. PMID: 11313476 Free PMC article.
-
Two novel Drosophila TAF(II)s have homology with human TAF(II)30 and are differentially regulated during development.Mol Cell Biol. 2000 Mar;20(5):1639-48. doi: 10.1128/MCB.20.5.1639-1648.2000. Mol Cell Biol. 2000. PMID: 10669741 Free PMC article.
Publication types
MeSH terms
Substances
Associated data
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Miscellaneous