Maternal-specific methylation of the imprinted mouse Igf2r locus identifies the expressed locus as carrying the imprinting signal
- PMID: 8462104
- DOI: 10.1016/0092-8674(93)90160-r
Maternal-specific methylation of the imprinted mouse Igf2r locus identifies the expressed locus as carrying the imprinting signal
Abstract
The mouse insulin-like growth factor type 2 receptor (Igf2r) is imprinted and expressed exclusively from the maternally inherited chromosome. To investigate whether methylation could function as the imprinting signal, we have cloned 130 kb from the Igf2r locus and searched for sequences methylated in a parental-specific manner. Two regions have been identified: region 1 contains the start of transcription and is methylated only on the silent paternal chromosome; region 2 is contained in an intron and is methylated only on the expressed maternal chromosome. Methylation of region 1 is acquired after fertilization, in contrast with the methylation of region 2, which is inherited from the female gamete. Methylation of region 2 may mark the maternal Igf2r locus in a manner that could act as an imprinting signal. These data suggest that the expressed locus carries a potential imprinting signal and imply that methylation is necessary for expression of the Igf2r gene.
Similar articles
-
Cross-species clues of an epigenetic imprinting regulatory code for the IGF2R gene.Cytogenet Genome Res. 2006;113(1-4):202-8. doi: 10.1159/000090833. Cytogenet Genome Res. 2006. PMID: 16575181 Review.
-
Imprinted expression of the Igf2r gene depends on an intronic CpG island.Nature. 1997 Oct 16;389(6652):745-9. doi: 10.1038/39631. Nature. 1997. PMID: 9338788
-
Maternal-specific methylation of the human IGF2R gene is not accompanied by allele-specific transcription.Genomics. 1996 Jan 15;31(2):158-66. doi: 10.1006/geno.1996.0027. Genomics. 1996. PMID: 8824797
-
Genomic imprinting in mice: its function and mechanism.Biol Reprod. 1996 Feb;54(2):273-8. doi: 10.1095/biolreprod54.2.273. Biol Reprod. 1996. PMID: 8788176 Review.
-
Conservation of a maternal-specific methylation signal at the human IGF2R locus.Hum Mol Genet. 1995 Oct;4(10):1945-52. doi: 10.1093/hmg/4.10.1945. Hum Mol Genet. 1995. PMID: 8595419
Cited by
-
Reshaping the Landscape of the Genome: Toolkits for Precise DNA Methylation Manipulation and Beyond.JACS Au. 2023 Dec 21;4(1):40-57. doi: 10.1021/jacsau.3c00671. eCollection 2024 Jan 22. JACS Au. 2023. PMID: 38274248 Free PMC article. Review.
-
The AIRN lncRNA is imprinted and paternally expressed in pigs.J Anim Sci. 2023 Jan 3;101:skad367. doi: 10.1093/jas/skad367. J Anim Sci. 2023. PMID: 37925372
-
Methylation is maintained specifically at imprinting control regions but not other DMRs associated with imprinted genes in mice bearing a mutation in the Dnmt1 intrinsically disordered domain.Front Cell Dev Biol. 2023 Aug 4;11:1192789. doi: 10.3389/fcell.2023.1192789. eCollection 2023. Front Cell Dev Biol. 2023. PMID: 37601113 Free PMC article.
-
Effects of 5-azacytidine on the development of parthenogenetic mouse embryos.Dev Growth Differ. 1996 Jun;38(3):263-270. doi: 10.1046/j.1440-169X.1996.t01-2-00005.x. Dev Growth Differ. 1996. PMID: 37281506
-
Placental imprinting of SLC22A3 in the IGF2R imprinted domain is conserved in therian mammals.Epigenetics Chromatin. 2022 Aug 27;15(1):32. doi: 10.1186/s13072-022-00465-4. Epigenetics Chromatin. 2022. PMID: 36030241 Free PMC article.
Publication types
MeSH terms
Substances
Associated data
- Actions
- Actions
- Actions
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Miscellaneous
