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. 1993 Apr;175(8):2436-44.
doi: 10.1128/jb.175.8.2436-2444.1993.

Sequence of the gene coding for ammonia monooxygenase in Nitrosomonas europaea

H McTavish  1 , J A Fuchs, A B Hooper
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Sequence of the gene coding for ammonia monooxygenase in Nitrosomonas europaea

H McTavish et al. J Bacteriol. 1993 Apr.

Abstract

Nitrosomonas europaea, a chemolithotrophic bacterium, was found to contain two copies of the gene coding for the presumed active site polypeptide of ammonia monooxygenase, the 32-kDa acetylene-binding polypeptide. One copy of this gene was cloned, and its complete nucleotide sequence is presented. Immediately downstream of this gene, in the same operon, is the gene for a 40-kDa polypeptide that copurifies with the ammonia monooxygenase acetylene-binding polypeptide. The sequence of the first 692 nucleotides of this structural gene, coding for about two-thirds of the protein, is presented. These sequences are the first sequences of protein-encoding genes from an ammonia-oxidizing autotrophic nitrifying bacterium. The two protein sequences are not homologous with the sequences of any other monooxygenase. From radioactive labelling of ammonia monooxygenase with [14C]acetylene it was determined that there are 23 nmol of ammonia monooxygenase per g of cells. The kcat of ammonia monooxygenase for NH3 in vivo was calculated to be 20 s-1.

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References

    1. Anal Biochem. 1990 Nov 1;190(2):348-53 - PubMed
    1. J Biol Chem. 1986 Dec 25;261(36):17048-56 - PubMed
    1. Biochemistry. 1989 Dec 26;28(26):10054-61 - PubMed
    1. Biochem J. 1985 May 1;227(3):719-25 - PubMed
    1. Biochemistry. 1991 Dec 3;30(48):11466-72 - PubMed

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