Transcriptional regulation of the dihydrofolate reductase gene
- PMID: 8593164
- DOI: 10.1002/bies.950180111
Transcriptional regulation of the dihydrofolate reductase gene
Abstract
As cells approach S phase, many changes occur to create an environment conducive for DNA synthesis and commitment to cell division. The transcription rate of many genes encoding enzymes involved in DNA synthesis, including the dihydrofolate reductase (dhfr) gene, increases at the G1/S boundary of the cell cycle. Although a number of transcription factors interact to finely tune the levels of dhfr RNA produced, two families of transcription factors, Sp1 and E2F, play central roles in modulating dhfr levels. A region containing several Sp1-binding sites is required for both regulated and basal transcription levels. In contrast, the E2F-binding sites near the transcription start site are required only for regulated transcription. A model is presented for the regulation of the dhfr gene which may also pertain to other cell cycle-associated genes.
Similar articles
-
The bidirectionally transcribed dihydrofolate reductase and rep-3a promoters are growth regulated by distinct mechanisms.Cell Growth Differ. 1995 May;6(5):541-8. Cell Growth Differ. 1995. PMID: 7647037
-
Transcription from TATA-less promoters: dihydrofolate reductase as a model.Crit Rev Eukaryot Gene Expr. 1993;3(4):229-54. Crit Rev Eukaryot Gene Expr. 1993. PMID: 8286846 Review.
-
Regulation of dihydrofolate reductase gene expression and E2F components in human diploid fibroblasts during growth and senescence.J Cell Physiol. 1996 Sep;168(3):580-8. doi: 10.1002/(SICI)1097-4652(199609)168:3<580::AID-JCP10>3.0.CO;2-3. J Cell Physiol. 1996. PMID: 8816912
-
Retinoblastoma protein associates with SP1 and activates the hamster dihydrofolate reductase promoter.Oncogene. 1998 Apr 16;16(15):1931-8. doi: 10.1038/sj.onc.1201718. Oncogene. 1998. PMID: 9591776
-
Transcriptional regulation of the dihydrofolate reductase/rep-3 locus.Crit Rev Eukaryot Gene Expr. 1994;4(1):19-53. doi: 10.1615/critreveukargeneexpr.v4.i1.20. Crit Rev Eukaryot Gene Expr. 1994. PMID: 7987046 Review.
Cited by
-
Initiator-Directed Transcription: Fission Yeast Nmtl Initiator Directs Preinitiation Complex Formation and Transcriptional Initiation.Genes (Basel). 2022 Jan 28;13(2):256. doi: 10.3390/genes13020256. Genes (Basel). 2022. PMID: 35205301 Free PMC article.
-
Combined Transcriptome Analysis Reveals the Ovule Abortion Regulatory Mechanisms in the Female Sterile Line of Pinus tabuliformis Carr.Int J Mol Sci. 2021 Mar 19;22(6):3138. doi: 10.3390/ijms22063138. Int J Mol Sci. 2021. PMID: 33808669 Free PMC article.
-
PPARδ agonist prevents endothelial dysfunction via induction of dihydrofolate reductase gene and activation of tetrahydrobiopterin salvage pathway.Br J Pharmacol. 2019 Aug;176(16):2945-2961. doi: 10.1111/bph.14745. Epub 2019 Jul 6. Br J Pharmacol. 2019. PMID: 31144304 Free PMC article.
-
CHIP E3 ligase mediates proteasomal degradation of the proliferation regulatory protein ALDH1L1 during the transition of NIH3T3 fibroblasts from G0/G1 to S-phase.PLoS One. 2018 Jul 6;13(7):e0199699. doi: 10.1371/journal.pone.0199699. eCollection 2018. PLoS One. 2018. PMID: 29979702 Free PMC article.
-
Down-regulation of dihydrofolate reductase inhibits the growth of endothelial EA.hy926 cell through induction of G1 cell cycle arrest via up-regulating p53 and p21(waf1/cip1) expression.J Clin Biochem Nutr. 2016 Mar;58(2):105-13. doi: 10.3164/jcbn.15-64. Epub 2016 Feb 4. J Clin Biochem Nutr. 2016. PMID: 27013776 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Research Materials