Review
doi: 10.1007/978-1-4615-1899-0_45.
Site directed mutagenesis of the murine coronavirus spike protein. Effects on fusion
Affiliations
- PMID: 8830493
- DOI: 10.1007/978-1-4615-1899-0_45
Item in Clipboard
Review
Site directed mutagenesis of the murine coronavirus spike protein. Effects on fusion
Adv Exp Med Biol.
1995.
Abstract
Mutations were introduced in the transmembrane region of the spike protein of the murine coronavirus A59. The maturation of these mutant S proteins was not affected, they were all expressed at the cell surface, and became acylated, however some mutant S proteins did not induce cell-to-cell fusion. An I-->K change in the middle of the predicted transmembrane (TM) anchor and mutation of the first three cysteine residues of the TM domain resulted in a fusion-negative phenotype. We propose a model by which these data can be explained.
Similar articles
-
Effects of amino acid insertions in the cysteine-rich domain of the MHV-A59 spike protein on cell fusion.Adv Exp Med Biol. 2001;494:205-11. doi: 10.1007/978-1-4615-1325-4_33. Adv Exp Med Biol. 2001. PMID: 11774470 No abstract available.
-
Receptor specificity and receptor-induced conformational changes in mouse hepatitis virus spike glycoprotein.Adv Exp Med Biol. 2001;494:173-81. doi: 10.1007/978-1-4615-1325-4_29. Adv Exp Med Biol. 2001. PMID: 11774465 Review. No abstract available.
-
Coronavirus-induced membrane fusion requires the cysteine-rich domain in the spike protein.Virology. 2000 Mar 30;269(1):212-24. doi: 10.1006/viro.2000.0219. Virology. 2000. PMID: 10725213 Free PMC article.
-
[Structure and biological functions of the spike protein of mouse hepatitis virus].Uirusu. 1996 Dec;46(2):109-17. doi: 10.2222/jsv.46.109. Uirusu. 1996. PMID: 9123883 Review. Japanese. No abstract available.
-
Mutational analysis of the murine coronavirus spike protein: effect on cell-to-cell fusion.Virology. 1995 Dec 20;214(2):453-63. doi: 10.1006/viro.1995.0056. Virology. 1995. PMID: 8553547 Free PMC article.
Cited by
-
Structural insights into coronavirus entry.Adv Virus Res. 2019;105:93-116. doi: 10.1016/bs.aivir.2019.08.002. Epub 2019 Aug 22. Adv Virus Res. 2019. PMID: 31522710 Free PMC article.
-
Crystal structure of the severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein dimerization domain reveals evolutionary linkage between corona- and arteriviridae.J Biol Chem. 2006 Jun 23;281(25):17134-17139. doi: 10.1074/jbc.M602107200. Epub 2006 Apr 20. J Biol Chem. 2006. PMID: 16627473 Free PMC article.