Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1996 Oct 29;93(22):12304-8.
doi: 10.1073/pnas.93.22.12304.

Cytoplasm-to-vacuole targeting and autophagy employ the same machinery to deliver proteins to the yeast vacuole

S V Scott  1 , A Hefner-Gravink, K A Morano, T Noda, Y Ohsumi, D J Klionsky
Affiliations

Cytoplasm-to-vacuole targeting and autophagy employ the same machinery to deliver proteins to the yeast vacuole

S V Scott et al. Proc Natl Acad Sci U S A. .

Abstract

The vacuolar protein aminopeptidase I (API) uses a novel cytoplasm-to-vacuole targeting (Cvt) pathway. Complementation analysis of yeast mutants defective for cytoplasm-to-vacuole protein targeting (cvt) and autophagy (apg) revealed seven overlapping complementation groups between these two sets of mutants. In addition, all 14 apg complementation groups are defective in the delivery of API to the vacuole. Similarly, the majority of nonoverlapping cvt complementation groups appear to be at least partially defective in autophagy. Kinetic analyses of protein delivery rates indicate that autophagic protein uptake is induced by nitrogen starvation, whereas Cvt is a constitutive biosynthetic pathway. However, the machinery governing Cvt is affected by nitrogen starvation as targeting defects resulting from API overexpression can be rescued by induction of autophagy.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Mol Cell Biol. 1988 Nov;8(11):4936-48 - PubMed
    1. Cell Struct Funct. 1995 Dec;20(6):465-71 - PubMed
    1. EMBO J. 1989 Aug;8(8):2241-50 - PubMed
    1. J Biol Chem. 1990 Oct 25;265(30):18447-53 - PubMed
    1. Microbiol Rev. 1990 Sep;54(3):266-92 - PubMed

Publication types

LinkOut - more resources

-