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. 1997 Feb;33(3):165-75.
doi: 10.1016/s0166-3542(96)01012-1.

Antiviral effects of 6-diazo-5-oxo-L-norleucin on replication of herpes simplex virus type 1

J Cinatl  1 , J U Vogel, J Cinatl, H Kabickova, B Kornhuber, H W Doerr
Affiliations

Antiviral effects of 6-diazo-5-oxo-L-norleucin on replication of herpes simplex virus type 1

J Cinatl et al. Antiviral Res. 1997 Feb.

Abstract

An L-glutamine antagonist, 6-diazo-5-oxo-L-norleucin (L-DON), inhibits replication of vesicular stomatitis virus, poliovirus and paramyxoviruses in cultured cells. We tested the antiviral activity of L-DON against different strains of herpes simplex virus type 1 (HSV-1) in Vero cells. In the presence of a physiological plasma concentration of L-glutamine (0.5mM) L-Don inhibited 50% production of virus plaques at concentrations ranging from 7.9 to 16 microM. At concentrations of 40 microM L-Don inhibited infectious virus yield by 99%. The antiviral activity of L-DON decreased with increasing L-glutamine concentrations. A concentration of 5000 microM of L-Don had no significant effects on the viability of Vero cells. Transmission electron microscopical investigations showed that L-DON prevented mainly envelopment of viral nucleocapsids in the cytoplasm. The immunoprecipitation experiments demonstrated selective inhibition of synthesis of HSV-1 glycoproteins in L-DON treated cells. The results showed that L-DON inhibits HSV-1 replication at a late stage in the virus replication cycle, probably the cytoplasmic maturation of virions and subsequent virion egress from the cells.

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