Molecular cloning of mouse Doc2alpha and distribution of its mRNA in adult mouse brain
- PMID: 9073161
- DOI: 10.1016/s0169-328x(96)00198-2
Molecular cloning of mouse Doc2alpha and distribution of its mRNA in adult mouse brain
Abstract
We have previously isolated from a human brain cDNA library, a new protein having two C2-like domains which interact with Ca2+ and phospholipid, and named Doc2alpha. Doc2alpha is abundantly expressed in brain, where it is highly concentrated on the synaptic vesicle fraction, and is implicated in Ca2(+)-dependent exocytosis. We have isolated here a mouse Doc2alpha cDNA and determined the localization of its mRNA in adult mouse brain. The amino acid sequence of the mouse Doc2alpha cDNA is 92% identical with that of the human counterpart. Northern blot analysis and in situ hybridization on adult mouse brain sections have revealed that Doc2alpha is predominantly expressed in mouse brain, where it is expressed in neuronal cells, but not in non-neuronal cells. Doc2alpha is highly expressed in the olfactory bulb, cerebral cortex, hippocampus, amygdaloid complex, and ventromedial hypothalamus nucleus, but not in the cerebellum, caudate-putamen, or ventral thalamus. These results indicate that Doc2alpha is expressed heterogeneously in mouse brain, where it is predominantly expressed in neuronal cells, and suggest that Doc2alpha plays a specific role in the area where it is expressed.
Similar articles
-
Role of the polybasic sequence in the Doc2alpha C2B domain in dense-core vesicle exocytosis in PC12 cells.J Neurochem. 2010 Jul;114(1):171-81. doi: 10.1111/j.1471-4159.2010.06739.x. Epub 2010 Apr 9. J Neurochem. 2010. PMID: 20403080
-
Doc2 alpha and Munc13-4 regulate Ca(2+) -dependent secretory lysosome exocytosis in mast cells.J Immunol. 2008 Apr 1;180(7):4774-84. doi: 10.4049/jimmunol.180.7.4774. J Immunol. 2008. PMID: 18354201
-
Doc2gamma, a third isoform of double C2 protein, lacking calcium-dependent phospholipid binding activity.Biochem Biophys Res Commun. 2000 Sep 24;276(2):626-32. doi: 10.1006/bbrc.2000.3520. Biochem Biophys Res Commun. 2000. PMID: 11027523
-
Identification of the novel developmentally regulated gene, Bdm2, which is highly expressed in fetal rat brain.Brain Res Dev Brain Res. 2000 Mar 15;120(1):57-64. doi: 10.1016/s0165-3806(99)00191-1. Brain Res Dev Brain Res. 2000. PMID: 10727730
-
Doc2: a novel brain protein having two repeated C2-like domains.Biochem Biophys Res Commun. 1995 Jan 17;206(2):439-48. doi: 10.1006/bbrc.1995.1062. Biochem Biophys Res Commun. 1995. PMID: 7826360
Cited by
-
Phosphorylation of Doc2 by EphB2 modulates Munc13-mediated SNARE complex assembly and neurotransmitter release.Sci Adv. 2024 May 17;10(20):eadi7024. doi: 10.1126/sciadv.adi7024. Epub 2024 May 17. Sci Adv. 2024. PMID: 38758791 Free PMC article.
-
DOC2B promotes insulin sensitivity in mice via a novel KLC1-dependent mechanism in skeletal muscle.Diabetologia. 2019 May;62(5):845-859. doi: 10.1007/s00125-019-4824-2. Epub 2019 Feb 1. Diabetologia. 2019. PMID: 30707251 Free PMC article.
-
Doc2 is a Ca2+ sensor required for asynchronous neurotransmitter release.Cell. 2011 Oct 28;147(3):666-77. doi: 10.1016/j.cell.2011.09.046. Cell. 2011. PMID: 22036572 Free PMC article.
-
The C2A domain of synaptotagmin-like protein 3 (Slp3) is an atypical calcium-dependent phospholipid-binding machine: comparison with the C2A domain of synaptotagmin I.Biochem J. 2002 Sep 1;366(Pt 2):681-7. doi: 10.1042/BJ20020484. Biochem J. 2002. PMID: 12049610 Free PMC article.
-
Is double C2 protein (DOC2) expressed in bovine adrenal medulla? A commercial anti-DOC2 monoclonal antibody recognizes a major bovine mitochondrial antigen.Biochem J. 2000 Oct 1;351(Pt 1):33-7. doi: 10.1042/0264-6021:3510033. Biochem J. 2000. PMID: 10998344 Free PMC article.
Publication types
MeSH terms
Substances
Associated data
- Actions
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Miscellaneous