mRNA silencing in erythroid differentiation: hnRNP K and hnRNP E1 regulate 15-lipoxygenase translation from the 3' end
- PMID: 9160751
- DOI: 10.1016/s0092-8674(00)80241-x
mRNA silencing in erythroid differentiation: hnRNP K and hnRNP E1 regulate 15-lipoxygenase translation from the 3' end
Abstract
Although LOX mRNA accumulates early during differentiation, a differentiation control element in its 3' untranslated region confers translational silencing until late stage erythropoiesis. We have purified two proteins from rabbit reticulocytes that specifically mediate LOX silencing and identified them as hnRNPs K and E1. Transfection of hnRNP K and hnRNP E1 into HeLa cells specifically silenced the translation of reporter mRNAs bearing a differentiation control element in their 3' untranslated region. Silenced LOX mRNA in rabbit reticulocytes specifically coimmunoprecipitated with hnRNP K. In a reconstituted cell-free translation system, addition of recombinant hnRNP K and hnRNP E1 recapitulates this regulation via a specific inhibition of 80S ribosome assembly on LOX mRNA. Both proteins can control cap-dependent and internal ribosome entry site-mediated translation by binding to differentiation control elements. Our data suggest a specific cytoplasmic function for hnRNPs as translational regulatory proteins.
Similar articles
-
DDX6 recruits translational silenced human reticulocyte 15-lipoxygenase mRNA to RNP granules.RNA. 2010 Nov;16(11):2189-204. doi: 10.1261/rna.2211110. Epub 2010 Sep 30. RNA. 2010. PMID: 20884783 Free PMC article.
-
Caspase-3 cleaves hnRNP K in erythroid differentiation.Cell Death Dis. 2013 Mar 21;4(3):e548. doi: 10.1038/cddis.2013.75. Cell Death Dis. 2013. PMID: 23519117 Free PMC article.
-
c-Src-mediated phosphorylation of hnRNP K drives translational activation of specifically silenced mRNAs.Mol Cell Biol. 2002 Jul;22(13):4535-43. doi: 10.1128/MCB.22.13.4535-4543.2002. Mol Cell Biol. 2002. PMID: 12052863 Free PMC article.
-
Control of mRNA translation and stability in haematopoietic cells: the function of hnRNPs K and E1/E2.Biol Cell. 2004 Aug;96(6):407-11. doi: 10.1016/j.biolcel.2004.03.010. Biol Cell. 2004. PMID: 15384226 Review.
-
Precision mechanics with multifunctional tools: how hnRNP K and hnRNPs E1/E2 contribute to post-transcriptional control of gene expression in hematopoiesis.Curr Protein Pept Sci. 2012 Jun;13(4):391-400. doi: 10.2174/138920312801619484. Curr Protein Pept Sci. 2012. PMID: 22708489 Review.
Cited by
-
Heterogeneous nuclear ribonucleoprotein K promotes cap-independent translation initiation of retroviral mRNAs.Nucleic Acids Res. 2024 Mar 21;52(5):2625-2647. doi: 10.1093/nar/gkad1221. Nucleic Acids Res. 2024. PMID: 38165048 Free PMC article.
-
The 3'‑untranslated region of XB130 regulates its mRNA stability and translational efficiency in non‑small cell lung cancer cells.Oncol Lett. 2023 Aug 17;26(4):427. doi: 10.3892/ol.2023.14013. eCollection 2023 Oct. Oncol Lett. 2023. PMID: 37720672 Free PMC article.
-
Functional Characterization of Transgenic Mice Overexpressing Human 15-Lipoxygenase-1 (ALOX15) under the Control of the aP2 Promoter.Int J Mol Sci. 2023 Mar 2;24(5):4815. doi: 10.3390/ijms24054815. Int J Mol Sci. 2023. PMID: 36902243 Free PMC article.
-
Peroxiredoxin 5 regulates osteogenic differentiation through interaction with hnRNPK during bone regeneration.Elife. 2023 Feb 3;12:e80122. doi: 10.7554/eLife.80122. Elife. 2023. PMID: 36735291 Free PMC article.
-
Non-Coding RNAs Are Implicit in Chronic Myeloid Leukemia Therapy Resistance.Int J Mol Sci. 2022 Oct 14;23(20):12271. doi: 10.3390/ijms232012271. Int J Mol Sci. 2022. PMID: 36293127 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous
