Effects of carbonylcyanide-m-chlorophenylhydrazone (CCCP) and acetate on Escherichia coli O157:H7 and K-12: uncoupling versus anion accumulation
- PMID: 9198284
- DOI: 10.1016/s0378-1097(97)00140-7
Effects of carbonylcyanide-m-chlorophenylhydrazone (CCCP) and acetate on Escherichia coli O157:H7 and K-12: uncoupling versus anion accumulation
Abstract
Non-growing cells of Escherichia coli O157:H7 and K-12 that were incubated anaerobically in sodium phosphate buffer at pH 6.5 consumed glucose at a rate of approximately 8 mumol.(mg protein)-1.h-1 and had intracellular pH values of 7.3 and 7.5, respectively. The uncoupler, carbonylcyanide-m-chlorophenylhydrazone (CCCP), caused a marked decrease in intracellular pH, ATP and potassium of both strains. Low concentrations of CCCP stimulated glucose consumption rate, but higher concentrations were inhibitory. Acetate also caused a decrease in intracellular pH, but it never caused a large decrease in glucose consumption rate. Acetate decreased the intracellular ATP of E. coli K-12, but it had no effect on the ATP of O157:H7. Acetate had no effect on the intracellular potassium of E. coli O157:H7, and acetate-treated K-12 cells had even more potassium than untreated controls. Based on these results, acetate and CCCP appear to have different effects on E. coli. The comparison of E. coli O157:H7 and K-12 indicated that intracellular pH, acetate accumulation and intracellular potassium were related. E. coli K-12 maintained a higher intracellular pH than O157:H7, accumulated more acetate and had a greater intracellular potassium.
Similar articles
-
The effects of fermentation acids on bacterial growth.Adv Microb Physiol. 1998;39:205-34. doi: 10.1016/s0065-2911(08)60017-x. Adv Microb Physiol. 1998. PMID: 9328648 Review.
-
The ability of Escherichia coli O157:H7 to decrease its intracellular pH and resist the toxicity of acetic acid.Microbiology (Reading). 1997 Apr;143 ( Pt 4):1175-1180. doi: 10.1099/00221287-143-4-1175. Microbiology (Reading). 1997. PMID: 9141680
-
Bacterial resistance to uncouplers.J Bioenerg Biomembr. 1994 Dec;26(6):639-46. doi: 10.1007/BF00831539. J Bioenerg Biomembr. 1994. PMID: 7721726 Review.
-
Lethal effect of carbonyl cyanide m-chlorophenylhydrazone on Escherichia coli and a halotolerant Brevibacterium species.Microbios. 1995;81(327):73-83. Microbios. 1995. PMID: 7476556
-
The effect of the uncoupler carbonyl cyanide m-chlorophenylhydrazone on K+ transport, ATP level and intracellular pH of Chlorella fusca.Biochim Biophys Acta. 1981 Jun 12;636(1):98-103. doi: 10.1016/0005-2728(81)90080-3. Biochim Biophys Acta. 1981. PMID: 7284347
Cited by
-
Salmonella enterica Infections Are Disrupted by Two Small Molecules That Accumulate within Phagosomes and Differentially Damage Bacterial Inner Membranes.mBio. 2022 Oct 26;13(5):e0179022. doi: 10.1128/mbio.01790-22. Epub 2022 Sep 22. mBio. 2022. PMID: 36135367 Free PMC article.
-
TBAJ-876 Displays Bedaquiline-Like Mycobactericidal Potency without Retaining the Parental Drug's Uncoupler Activity.Antimicrob Agents Chemother. 2020 Jan 27;64(2):e01540-19. doi: 10.1128/AAC.01540-19. Print 2020 Jan 27. Antimicrob Agents Chemother. 2020. PMID: 31712198 Free PMC article.
-
Acetate Metabolism and the Inhibition of Bacterial Growth by Acetate.J Bacteriol. 2019 Jun 10;201(13):e00147-19. doi: 10.1128/JB.00147-19. Print 2019 Jul 1. J Bacteriol. 2019. PMID: 30988035 Free PMC article.
-
Experimental Evolution of Escherichia coli K-12 in the Presence of Proton Motive Force (PMF) Uncoupler Carbonyl Cyanide m-Chlorophenylhydrazone Selects for Mutations Affecting PMF-Driven Drug Efflux Pumps.Appl Environ Microbiol. 2019 Feb 20;85(5):e02792-18. doi: 10.1128/AEM.02792-18. Print 2019 Mar 1. Appl Environ Microbiol. 2019. PMID: 30578262 Free PMC article.
-
Heterogeneous localisation of membrane proteins in Staphylococcus aureus.Sci Rep. 2018 Feb 26;8(1):3657. doi: 10.1038/s41598-018-21750-x. Sci Rep. 2018. PMID: 29483609 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Research Materials