Control of actin dynamics in cell motility
- PMID: 9217250
- DOI: 10.1006/jmbi.1997.1062
Control of actin dynamics in cell motility
Abstract
Actin polymerization plays a major role in cell movement. The controls of actin sequestration/desequestration and of filament turnover are two important features of cell motility. Actin binding proteins use properties derived from the steady-state monomer-polymer cycle of actin in the presence of ATP, to control the F-actin/G-actin ratio and the turnover rate of actin filaments. Capping proteins and profilin regulate the size of the pools of F-actin and unassembled actin by affecting the steady-state concentration of ATP-G-actin. At steady state, the treadmilling cycle of actin filaments is fed by their disassembly from the pointed ends. It is regulated in two different ways by capping proteins and ADF, as follows. Capping proteins, in decreasing the number of growing barbed ends, increase their individual rate of growth and create a "funneled" treadmilling process. ADF/cofilin, in increasing the rate of pointed-end disassembly, increases the rate of filament turnover, hence the rate of barbed-end growth. In conclusion, capping proteins and ADF cooperate to increase the rate of actin assembly up to values that support the rates of actin-based motility processes.
Similar articles
-
Determining the differences in actin binding by human ADF and cofilin.J Mol Biol. 2002 Jan 25;315(4):911-25. doi: 10.1006/jmbi.2001.5280. J Mol Biol. 2002. PMID: 11812157
-
The Arp2/3 complex branches filament barbed ends: functional antagonism with capping proteins.Nat Cell Biol. 2000 Jul;2(7):385-91. doi: 10.1038/35017011. Nat Cell Biol. 2000. PMID: 10878802
-
Actin depolymerizing factor (ADF/cofilin) enhances the rate of filament turnover: implication in actin-based motility.J Cell Biol. 1997 Mar 24;136(6):1307-22. doi: 10.1083/jcb.136.6.1307. J Cell Biol. 1997. PMID: 9087445 Free PMC article.
-
Beginning and ending an actin filament: control at the barbed end.Curr Top Dev Biol. 2004;63:145-88. doi: 10.1016/S0070-2153(04)63005-5. Curr Top Dev Biol. 2004. PMID: 15536016 Review.
-
Regulation of actin filament dynamics by actin depolymerizing factor/cofilin and actin-interacting protein 1: new blades for twisted filaments.Biochemistry. 2003 Nov 25;42(46):13363-70. doi: 10.1021/bi034600x. Biochemistry. 2003. PMID: 14621980 Review.
Cited by
-
Measurement and Characterization of the Electrical Properties of Actin Filaments.Int J Mol Sci. 2024 May 17;25(10):5485. doi: 10.3390/ijms25105485. Int J Mol Sci. 2024. PMID: 38791524 Free PMC article.
-
Bsp1, a fungal CPI motif protein, regulates actin filament capping in endocytosis and cytokinesis.Mol Biol Cell. 2024 Feb 1;35(2):br6. doi: 10.1091/mbc.E23-10-0391. Epub 2023 Dec 13. Mol Biol Cell. 2024. PMID: 38088874 Free PMC article.
-
The intrinsically disordered cytoplasmic tail of a dendrite branching receptor uses two distinct mechanisms to regulate the actin cytoskeleton.Elife. 2023 Aug 9;12:e88492. doi: 10.7554/eLife.88492. Elife. 2023. PMID: 37555826 Free PMC article.
-
Developmentally regulated mitochondrial biogenesis and cell death competence in maize pollen.BMC Plant Biol. 2022 Nov 1;22(1):508. doi: 10.1186/s12870-022-03897-y. BMC Plant Biol. 2022. PMID: 36316635 Free PMC article.
-
Numerical analysis of the impact of cytoskeletal actin filament density alterations onto the diffusive vesicle-mediated cell transport.PLoS Comput Biol. 2021 May 3;17(5):e1008784. doi: 10.1371/journal.pcbi.1008784. eCollection 2021 May. PLoS Comput Biol. 2021. PMID: 33939706 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials