The N-terminal region of the luteovirus readthrough domain determines virus binding to Buchnera GroEL and is essential for virus persistence in the aphid
- PMID: 9311800
- PMCID: PMC192067
- DOI: 10.1128/JVI.71.10.7258-7265.1997
The N-terminal region of the luteovirus readthrough domain determines virus binding to Buchnera GroEL and is essential for virus persistence in the aphid
Abstract
Luteoviruses and the luteovirus-like pea enation mosaic virus (PEMV; genus Enamovirus) are transmitted by aphids in a circulative, nonreplicative manner. Acquired virus particles persist for several weeks in the aphid hemolymph, in which a GroEL homolog, produced by the primary endosymbiont of the aphid, is abundantly present. Six subgroup II luteoviruses and PEMV displayed a specific but differential affinity for Escherichia coli GroEL and GroEL homologs isolated from the endosymbiotic bacteria of both vector and nonvector aphid species. These observations suggest that the basic virus-binding capacity resides in a conserved region of the GroEL molecule, although other GroEL domains may influence the efficiency of binding. Purified luteovirus and enamovirus particles contain a major 22-kDa coat protein (CP) and lesser amounts of an approximately 54-kDa readthrough protein, expressed by translational readthrough of the CP into the adjacent open reading frame. Beet western yellows luteovirus (BWYV) mutants devoid of the readthrough domain (RTD) did not bind to Buchnera GroEL, demonstrating that the RTD (and not the highly conserved CP) contains the determinants for GroEL binding. In vivo studies showed that virions of these BWYV mutants were significantly less persistent in the aphid hemolymph than were virions containing the readthrough protein. These data suggest that the Buchnera GroEL-RTD interaction protects the virus from rapid degradation in the aphid. Sequence comparison analysis of the RTDs of different luteoviruses and PEMV identified conserved residues potentially important in the interaction with Buchnera GroEL.
Similar articles
-
Potato leafroll virus binds to the equatorial domain of the aphid endosymbiotic GroEL homolog.J Virol. 1998 Jan;72(1):358-65. doi: 10.1128/JVI.72.1.358-365.1998. J Virol. 1998. PMID: 9420234 Free PMC article.
-
Interactions between a luteovirus and the GroEL chaperonin protein of the symbiotic bacterium Buchnera aphidicola of aphids.J Gen Virol. 2011 Jun;92(Pt 6):1467-1474. doi: 10.1099/vir.0.029355-0. Epub 2011 Feb 23. J Gen Virol. 2011. PMID: 21346031
-
Effects of mutations in the beet western yellows virus readthrough protein on its expression and packaging and on virus accumulation, symptoms, and aphid transmission.Virology. 1997 Apr 14;230(2):323-34. doi: 10.1006/viro.1997.8476. Virology. 1997. PMID: 9143288
-
Enhancing Capsid Proteins Capacity in Plant Virus-Vector Interactions and Virus Transmission.Cells. 2021 Jan 7;10(1):90. doi: 10.3390/cells10010090. Cells. 2021. PMID: 33430410 Free PMC article. Review.
-
Pea enation mosaic and the vagaries of a plant virus.Adv Virus Res. 2001;57:323-50. doi: 10.1016/s0065-3527(01)57007-4. Adv Virus Res. 2001. PMID: 11680388 Review. No abstract available.
Cited by
-
Genomic variation in pepper vein yellows viruses in Australia, including a new putative variant, PeVYV-10.Arch Virol. 2024 Jan 5;169(1):18. doi: 10.1007/s00705-023-05943-y. Arch Virol. 2024. PMID: 38180533
-
Molecular Characterization of a Novel Enamovirus Infecting Raspberry.Viruses. 2023 Nov 21;15(12):2281. doi: 10.3390/v15122281. Viruses. 2023. PMID: 38140523 Free PMC article.
-
Small Talk: On the Possible Role of Trans-Kingdom Small RNAs during Plant-Virus-Vector Tritrophic Communication.Plants (Basel). 2023 Mar 22;12(6):1411. doi: 10.3390/plants12061411. Plants (Basel). 2023. PMID: 36987098 Free PMC article. Review.
-
Proteomic and Transcriptomic Analysis for Identification of Endosymbiotic Bacteria Associated with BYDV Transmission Efficiency by Sitobion miscanthi.Plants (Basel). 2022 Dec 2;11(23):3352. doi: 10.3390/plants11233352. Plants (Basel). 2022. PMID: 36501390 Free PMC article.
-
Weed Hosts Represent an Important Reservoir of Turnip Yellows Virus and a Possible Source of Virus Introduction into Oilseed Rape Crop.Viruses. 2022 Nov 13;14(11):2511. doi: 10.3390/v14112511. Viruses. 2022. PMID: 36423120 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
Miscellaneous