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. 1997 Dec;17(12):6822-30.
doi: 10.1128/MCB.17.12.6822.

Initiation of DNA interstrand cross-link repair in humans: the nucleotide excision repair system makes dual incisions 5' to the cross-linked base and removes a 22- to 28-nucleotide-long damage-free strand

T Bessho  1 , D Mu, A Sancar
Affiliations

Initiation of DNA interstrand cross-link repair in humans: the nucleotide excision repair system makes dual incisions 5' to the cross-linked base and removes a 22- to 28-nucleotide-long damage-free strand

T Bessho et al. Mol Cell Biol. 1997 Dec.

Abstract

Most DNA repair mechanisms rely on the redundant information inherent to the duplex to remove damaged nucleotides and replace them with normal ones, using the complementary strand as a template. Interstrand cross-links pose a unique challenge to the DNA repair machinery because both strands are damaged. To study the repair of interstrand cross-links by mammalian cells, we tested the activities of cell extracts of wild-type or excision repair-defective rodent cell lines and of purified human excision nuclease on a duplex with a site-specific cross-link. We found that in contrast to monoadducts, which are removed by dual incisions bracketing the lesion, the cross-link causes dual incisions, both 5' to the cross-link in one of the two strands. The net result is the generation of a 22- to 28-nucleotide-long gap immediately 5' to the cross-link. This gap may act as a recombinogenic signal to initiate cross-link removal.

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References

    1. Proc Natl Acad Sci U S A. 1982 Mar;79(6):1722-6 - PubMed
    1. J Biol Chem. 1997 Mar 21;272(12):7570-3 - PubMed
    1. J Biol Chem. 1989 Apr 25;264(12):6755-65 - PubMed
    1. Mol Cell Biol. 1989 Jul;9(7):2897-905 - PubMed
    1. J Biol Chem. 1990 Feb 25;265(6):3489-96 - PubMed

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