Molecular evolution of amphioxus fructose-1,6-bisphosphate aldolase
- PMID: 9434745
- DOI: 10.1006/abbi.1997.0384
Molecular evolution of amphioxus fructose-1,6-bisphosphate aldolase
Abstract
The cDNA for amphioxus fructose-1,6-bisphosphate (FBP)-aldolase was isolated and its nucleotide sequence was determined. In the cDNA, there existed a probable open reading frame comprising 1080 bp; hence, 359 amino acid residues were deduced. The amino acid sequence indicates the deletion of 4 residues from N-terminus, in comparison with the sequence of FBP-aldolase isozymes from other sources. There was only one FBP-aldolase gene, and one enzyme species corresponding, in the amphioxus; this is the first report of the existence of a single FBP-aldolase species in animals. Enzymatic studies of both native and the recombinant FBP-aldolase suggest that the amphioxus enzyme belongs to an ancestral class I type which is not discovered among vertebrate aldolase isozymes.
Similar articles
-
Caenorhabditis elegans has two isozymic forms, CE-1 and CE-2, of fructose-1,6-bisphosphate aldolase which are encoded by different genes.Arch Biochem Biophys. 1997 Mar 1;339(1):226-34. doi: 10.1006/abbi.1996.9813. Arch Biochem Biophys. 1997. PMID: 9056253
-
Molecular cloning, expression, purification, and characterization of fructose-1,6-bisphosphate aldolase from Thermus aquaticus.Protein Expr Purif. 2001 Mar;21(2):293-302. doi: 10.1006/prep.2000.1380. Protein Expr Purif. 2001. PMID: 11237691
-
Characterization, expression and localization of S-adenosylhomocysteine hydrolase from amphioxus Branchiostoma belcheri tsingtaunese.Biosci Rep. 2008 Jun;28(3):135-44. doi: 10.1042/BSR20080024. Biosci Rep. 2008. PMID: 18532926
-
Structure and expression of human and rat aldolase isozyme genes: multiple mRNA species of aldolase A produced from a single gene.Isozymes Curr Top Biol Med Res. 1987;14:153-75. Isozymes Curr Top Biol Med Res. 1987. PMID: 3298145 Review. No abstract available.
-
Aldolase gene and protein families: structure, expression and pathophysiology.Horiz Biochem Biophys. 1986;8:611-65. Horiz Biochem Biophys. 1986. PMID: 3530956 Review. No abstract available.
Cited by
-
Comparative EST analysis provides insights into the basal aquatic fungus Blastocladiella emersonii.BMC Genomics. 2006 Jul 12;7:177. doi: 10.1186/1471-2164-7-177. BMC Genomics. 2006. PMID: 16836762 Free PMC article.
-
Expressed sequence tag analysis of adult Clonorchis sinensis, the Chinese liver fluke.Parasitol Res. 2006 Oct;99(5):602-8. doi: 10.1007/s00436-006-0204-1. Epub 2006 May 17. Parasitol Res. 2006. PMID: 16705464
-
Were vertebrates octoploid?Philos Trans R Soc Lond B Biol Sci. 2002 Apr 29;357(1420):531-44. doi: 10.1098/rstb.2001.1035. Philos Trans R Soc Lond B Biol Sci. 2002. PMID: 12028790 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous
