Distribution of 11-cis LRAT, 11-cis RD and 11-cis REH in bovine retinal pigment epithelium membranes
- PMID: 9767084
- DOI: 10.1016/s0005-2760(98)00078-2
Distribution of 11-cis LRAT, 11-cis RD and 11-cis REH in bovine retinal pigment epithelium membranes
Abstract
Our recent finding of the co-localization of 11-cis retinyl esters and 11-cis retinyl ester hydrolase (11-cis REH) activity in bovine retinal pigment epithelium (RPE) plasma membrane (PM) has led us to explore the possibility that the PM may provide 11-cis retinal for rhodopsin regeneration. In the RPE, visual chromophore is synthesized via a membrane associated 11-cis retinol dehydrogenase (11-cis RD). Accordingly, bovine RPE membranes enriched with either endoplasmic reticulum (ER) or plasma membrane (PM) enzyme markers were prepared and assayed for visual cycle enzyme activities. Pronounced 11-cis RD activity was associated with both ER- and PM-enriched membrane fractions. In contrast, 11-cis REH activity was mostly recovered in PM-enriched fractions while LRAT activity was found only in ER-enriched membranes. The finding that both 11-cis retinol and 11-cis retinal can be produced at the PM of the bovine RPE strongly suggests that 11-cis retinyl esters at this subcellular locale serve as a precursor of visual chromophore for pigment regeneration.
Similar articles
-
Retinol Dehydrogenases Regulate Vitamin A Metabolism for Visual Function.Nutrients. 2016 Nov 22;8(11):746. doi: 10.3390/nu8110746. Nutrients. 2016. PMID: 27879662 Free PMC article. Review.
-
The oxidation of 9-cis-retinol: a possible synthesis pathway for 9-cis-retinoic acid.Nutr Rev. 2000 Nov;58(11):354-6. doi: 10.1111/j.1753-4887.2000.tb01833.x. Nutr Rev. 2000. PMID: 11140907 Review.
-
Colocalization of 11-cis retinyl esters and retinyl ester hydrolase activity in retinal pigment epithelium plasma membrane.Invest Ophthalmol Vis Sci. 1998 Jul;39(8):1312-9. Invest Ophthalmol Vis Sci. 1998. PMID: 9660478
-
Substrate specificity of retinyl ester hydrolase activity in retinal pigment epithelium.J Lipid Res. 1998 Mar;39(3):604-12. J Lipid Res. 1998. PMID: 9548592
-
Comparison of retinyl ester hydrolase activities in bovine liver and retinal pigment epithelium.J Lipid Res. 1996 Sep;37(9):1947-52. J Lipid Res. 1996. PMID: 8895060
Cited by
-
Light-Induced Smooth Endoplasmic Reticulum Rearrangement in a Unique Interlaced Compartmental Pattern in Macaca mulatta RPE.Invest Ophthalmol Vis Sci. 2021 Dec 1;62(15):32. doi: 10.1167/iovs.62.15.32. Invest Ophthalmol Vis Sci. 2021. PMID: 34967853 Free PMC article.
-
Conditional Ablation of Retinol Dehydrogenase 10 in the Retinal Pigmented Epithelium Causes Delayed Dark Adaption in Mice.J Biol Chem. 2015 Nov 6;290(45):27239-27247. doi: 10.1074/jbc.M115.682096. Epub 2015 Sep 21. J Biol Chem. 2015. PMID: 26391396 Free PMC article.
-
New developments in the visual cycle: functional role of 11-cis retinyl esters in the retinal pigment epithelium.Hong Kong J Ophthalmol. 2001 Oct;5(1):25-29. Hong Kong J Ophthalmol. 2001. PMID: 25750490 Free PMC article.
-
The cone-specific visual cycle.Prog Retin Eye Res. 2011 Mar;30(2):115-28. doi: 10.1016/j.preteyeres.2010.11.001. Epub 2010 Nov 25. Prog Retin Eye Res. 2011. PMID: 21111842 Free PMC article. Review.
-
A novel cone visual cycle in the cone-dominated retina.Exp Eye Res. 2007 Aug;85(2):175-84. doi: 10.1016/j.exer.2007.05.003. Epub 2007 May 24. Exp Eye Res. 2007. PMID: 17618621 Free PMC article. Review.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Medical
Molecular Biology Databases
Miscellaneous