The mouse mitotic checkpoint gene bub1b, a novel bub1 family member, is expressed in a cell cycle-dependent manner
- PMID: 9889005
- DOI: 10.1006/geno.1998.5629
The mouse mitotic checkpoint gene bub1b, a novel bub1 family member, is expressed in a cell cycle-dependent manner
Abstract
A search for genes differentially expressed in normal and leukemic mouse thymocytes yielded a homolog of the yeast mitotic checkpoint protein Bub1. This novel protein ("mBub1b") has 40% sequence similarity to the mouse Bub1 ("mBub1a") previously described by Taylor and McKeon (1997, Cell 89, 727-735) over four extended domains. Differences between the Bub1 sequences suggest that the two proteins may have different substrate specificities and that Bub1b alone has a putative "destruction" box that can target proteins for degradation by proteosomes during mitosis. Northern blots of normal tissues show that mouse Bub1a and Bub1b genes are expressed in thymus and spleen, but not in nondividing tissues. In synchronized cells, expression of both Bub1 genes is undetectable in G1; Bub1 gene expression peaks in G2/M with Bub1b delayed by 6 h relative to Bub1a. This cell cycle-dependent expression explains the tissue distribution and the abundance of Bub1 mRNAs in rapidly dividing cell lines. The human equivalent of mBub1b was isolated and mapped to chromosome 15q15. The existence in mammals of two separate Bub1 genes encoding distinct proteins, coupled with the different timing of peak expression, suggests that Bub1a and Bub1b have distinct roles in the mitotic checkpoint.
Copyright 1999 Academic Press.
Similar articles
-
Spindle checkpoint protein Bub1 corrects mitotic aberrancy induced by human T-cell leukemia virus type I Tax.Oncogene. 2006 Jun 22;25(26):3621-7. doi: 10.1038/sj.onc.1209404. Epub 2006 Jan 30. Oncogene. 2006. PMID: 16449967
-
Increased expression of mitotic checkpoint genes in breast cancer cells with chromosomal instability.Clin Cancer Res. 2006 Jan 15;12(2):405-10. doi: 10.1158/1078-0432.CCR-05-0903. Clin Cancer Res. 2006. PMID: 16428479
-
A double missense variation of the BUB1 gene and a defective mitotic spindle checkpoint in the pancreatic cancer cell line Hs766T.Hum Mutat. 2003 Apr;21(4):445. doi: 10.1002/humu.9120. Hum Mutat. 2003. PMID: 12655561
-
Human Bub1: a putative spindle checkpoint kinase closely linked to cell proliferation.Cell Growth Differ. 1998 Oct;9(10):877-85. Cell Growth Differ. 1998. PMID: 9790499
-
Mammalian BUB1 protein kinases: map positions and in vivo expression.Genomics. 1997 Dec 15;46(3):379-88. doi: 10.1006/geno.1997.5068. Genomics. 1997. PMID: 9441741
Cited by
-
Comparative transcriptomics analysis identifies crucial genes and pathways during goose spleen development.Front Immunol. 2024 Feb 5;15:1327166. doi: 10.3389/fimmu.2024.1327166. eCollection 2024. Front Immunol. 2024. PMID: 38375472 Free PMC article.
-
RBFOX2 regulated EYA3 isoforms partner with SIX4 or ZBTB1 to control transcription during myogenesis.iScience. 2023 Oct 20;26(11):108258. doi: 10.1016/j.isci.2023.108258. eCollection 2023 Nov 17. iScience. 2023. PMID: 38026174 Free PMC article.
-
Identification of the Key Genes Involved in the Tumorigenesis and Prognosis of Prostate Cancer.Comput Math Methods Med. 2022 Oct 5;2022:5500416. doi: 10.1155/2022/5500416. eCollection 2022. Comput Math Methods Med. 2022. PMID: 36245843 Free PMC article.
-
Bioinformatic identification of differentially expressed genes associated with hepatocellular carcinoma prognosis.Medicine (Baltimore). 2022 Sep 23;101(38):e30678. doi: 10.1097/MD.0000000000030678. Medicine (Baltimore). 2022. PMID: 36197270 Free PMC article.
-
BUB1B (BUB1 Mitotic Checkpoint Serine/Threonine Kinase B) promotes lung adenocarcinoma by interacting with Zinc Finger Protein ZNF143 and regulating glycolysis.Bioengineered. 2022 Feb;13(2):2471-2485. doi: 10.1080/21655979.2021.2013108. Bioengineered. 2022. PMID: 35068350 Free PMC article.
Publication types
MeSH terms
Substances
Associated data
- Actions
- Actions
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases