Intracellular localization and membrane topology of 11-cis retinol dehydrogenase in the retinal pigment epithelium suggest a compartmentalized synthesis of 11-cis retinaldehyde
- PMID: 9914166
- DOI: 10.1242/jcs.112.4.549
Intracellular localization and membrane topology of 11-cis retinol dehydrogenase in the retinal pigment epithelium suggest a compartmentalized synthesis of 11-cis retinaldehyde
Abstract
11-cis retinol dehydrogenase (EC 1.1.1.105) catalyses the last step in the biosynthetic pathway generating 11-cis retinaldehyde, the common chromophore of all visual pigments in higher animals. The enzyme is abundantly expressed in retinal pigment epithelium of the eye and is a member of the short chain dehydrogenase/reductase superfamily. In this work we demonstrate that a majority of 11-cis retinol dehydrogenase is associated with the smooth ER in retinal pigment epithelial cells and that the enzyme is an integral membrane protein, anchored to membranes by two hydrophobic peptide segments. The catalytic domain of the enzyme is confined to a lumenal compartment and is not present on the cytosolic aspect of membranes. Thus, the subcellular localization and the membrane topology of 11-cis retinol dehydrogenase suggest that generation of 11-cis retinaldehyde is a compartmentalized process.
Similar articles
-
Structure and function of retinol dehydrogenases of the short chain dehydrogenase/reductase family.Mol Aspects Med. 2003 Dec;24(6):403-9. doi: 10.1016/s0098-2997(03)00036-0. Mol Aspects Med. 2003. PMID: 14585311 Review.
-
The oxidation of 9-cis-retinol: a possible synthesis pathway for 9-cis-retinoic acid.Nutr Rev. 2000 Nov;58(11):354-6. doi: 10.1111/j.1753-4887.2000.tb01833.x. Nutr Rev. 2000. PMID: 11140907 Review.
-
Distribution of 11-cis LRAT, 11-cis RD and 11-cis REH in bovine retinal pigment epithelium membranes.Biochim Biophys Acta. 1998 Oct 2;1394(1):16-22. doi: 10.1016/s0005-2760(98)00078-2. Biochim Biophys Acta. 1998. PMID: 9767084
-
Identification and immunohistochemistry of retinol dehydrogenase from bovine retinal pigment epithelium.Biochim Biophys Acta. 1993 May 13;1163(2):201-8. doi: 10.1016/0167-4838(93)90182-q. Biochim Biophys Acta. 1993. PMID: 8490052
-
The retinal pigment epithelial-specific 11-cis retinol dehydrogenase belongs to the family of short chain alcohol dehydrogenases.J Biol Chem. 1995 Jan 20;270(3):1107-12. J Biol Chem. 1995. PMID: 7836368
Cited by
-
Cellular and Molecular Mechanisms of Pathogenesis Underlying Inherited Retinal Dystrophies.Biomolecules. 2023 Feb 1;13(2):271. doi: 10.3390/biom13020271. Biomolecules. 2023. PMID: 36830640 Free PMC article. Review.
-
Novel variants in the RDH5 Gene in a Chinese Han family with fundus albipunctatus.BMC Ophthalmol. 2022 Feb 11;22(1):69. doi: 10.1186/s12886-022-02301-5. BMC Ophthalmol. 2022. PMID: 35148716 Free PMC article.
-
Light-Induced Smooth Endoplasmic Reticulum Rearrangement in a Unique Interlaced Compartmental Pattern in Macaca mulatta RPE.Invest Ophthalmol Vis Sci. 2021 Dec 1;62(15):32. doi: 10.1167/iovs.62.15.32. Invest Ophthalmol Vis Sci. 2021. PMID: 34967853 Free PMC article.
-
A large animal model of RDH5-associated retinopathy recapitulates important features of the human phenotype.Hum Mol Genet. 2022 Apr 22;31(8):1263-1277. doi: 10.1093/hmg/ddab316. Hum Mol Genet. 2022. PMID: 34726233 Free PMC article.
-
A spectral-domain optical coherence tomographic analysis of Rdh5-/- mice retina.PLoS One. 2020 Apr 9;15(4):e0231220. doi: 10.1371/journal.pone.0231220. eCollection 2020. PLoS One. 2020. PMID: 32271812 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources