[HTML][HTML] Condensins, chromosome condensation protein complexes containing XCAP-C, XCAP-E and a Xenopus homolog of the Drosophila Barren protein
T Hirano, R Kobayashi, M Hirano - Cell, 1997 - cell.com
T Hirano, R Kobayashi, M Hirano
Cell, 1997•cell.comWe report here purification and characterization of chro-mosome condensation protein
complexes (termed condensins) containing XCAP-C and XCAP-E, two Xenopus members of
the SMC family. Sucrose density gradient centrifugation reveals two major forms of
condensins. The 8S form is a heterodimer of XCAP-C and XCAP-E, whereas the 13S form
contains three additional subunits. One of them is identified as a homolog of the Drosophila
Barren protein whose mutation shows a defect in chromosome segregation. Chromosomal …
complexes (termed condensins) containing XCAP-C and XCAP-E, two Xenopus members of
the SMC family. Sucrose density gradient centrifugation reveals two major forms of
condensins. The 8S form is a heterodimer of XCAP-C and XCAP-E, whereas the 13S form
contains three additional subunits. One of them is identified as a homolog of the Drosophila
Barren protein whose mutation shows a defect in chromosome segregation. Chromosomal …
Abstract
We report here purification and characterization of chro- mosome condensation protein complexes (termed condensins) containing XCAP-C and XCAP-E, two Xenopus members of the SMC family. Sucrose density gradient centrifugation reveals two major forms of condensins. The 8S form is a heterodimer of XCAP-C and XCAP-E, whereas the 13S form contains three additional subunits. One of them is identified as a homolog of the Drosophila Barren protein whose mutation shows a defect in chromosome segregation. Chromosomal targeting of condensins is mitosis-specific and is independent of topoisomerase IIα. 13S condensin is required for condensation, as demonstrated by immunodepletion and rescue experiments. Our results suggest that the condensin complexes represent the most abundant structural components of mitotic chromosomes and play a central role in driving chromosome condensation.
cell.com