Supplementary Figure 4: Visceral fat pads are covered by mesothelia and short tamoxifen-pulsing labeled mesothelia.
From: Visceral and subcutaneous fat have different origins and evidence supports a mesothelial source
(a) Visceral fat pads in adult mice are covered by mesothelia. Immunofluorescence staining of sections from epididymal, epicardial, mesenteric, and omental fat pads (from Wt1-GFP mice) with GFP (green) and cytokeratin (red, a marker for mesothelia) antibodies. DAPI is indicated in blue. (b)Short tamoxifen-pulsing labeled mesothelia in lineage tracing experiment. Immunohistochemistry using GFP (green) and cytokeratin (red) antibodies on samples from the region of GI tract in Wt1-CreERT2; mTmG embryos induced with tamoxifen at E14.5 and analysed at E16.5. Cell nuclei is stained with DAPI (blue).
