Supplementary Figure 4: Visceral fat pads are covered by mesothelia and short tamoxifen-pulsing labeled mesothelia.
From: Visceral and subcutaneous fat have different origins and evidence supports a mesothelial source
![Supplementary Figure 4](https://media.springernature.com/full/springer-static/image/art%3A10.1038%2Fncb2922/MediaObjects/41556_2014_Article_BFncb2922_Fig9_ESM.jpg)
(a) Visceral fat pads in adult mice are covered by mesothelia. Immunofluorescence staining of sections from epididymal, epicardial, mesenteric, and omental fat pads (from Wt1-GFP mice) with GFP (green) and cytokeratin (red, a marker for mesothelia) antibodies. DAPI is indicated in blue. (b)Short tamoxifen-pulsing labeled mesothelia in lineage tracing experiment. Immunohistochemistry using GFP (green) and cytokeratin (red) antibodies on samples from the region of GI tract in Wt1-CreERT2; mTmG embryos induced with tamoxifen at E14.5 and analysed at E16.5. Cell nuclei is stained with DAPI (blue).