Extended Data Fig. 4: Characterization of HFD-fed TFEBMac-Tg mice.
From: TFEB–GDF15 axis protects against obesity and insulin resistance as a lysosomal stress response
a, Representative gross images of adipose tissues from TFEBMac-Tg and control mice fed HFD for 12 weeks. b,c, Distribution of adipocyte size (diameter) in eWAT (b) and iWAT (c) of TFEBMac-Tg and control mice fed HFD for 12 weeks (n=4-6). d,e, Quantification of CLS (d) and F4/80 immunohistochemistry (e) in eWAT of TFEBMac-Tg and control mice fed HFD for 12 weeks (n=4-6). f, Relative expression of inflammatory markers in iWAT of TFEBMac-Tg and control mice fed HFD for 12 weeks (n=4-6). g, Average EE during day and night time periods in TFEBMac-Tg and control mice fed HFD for 8 weeks (n=8-10). h,i, Average food intake (h) and locomotor activity (i) during day and night time periods in TFEBMac-Tg and control mice fed HFD for 8 weeks (n=8-10). j-n, T3 (j), T4 (k), fibroblast growth factor 21 (FGF21) (l), adiponectin (m) and leptin (n) levels in TFEBMac-Tg and control mice fed HFD for 12 weeks (n=8-15). o, serum GDF15 in TFEBMac-Con/Atg7W/W, TFEBMac-Tg/Atg7W/W, TFEBMac-Con/Atg7DMac and TFEBMac-Tg/Atg7DMac mice fed HFD for 12 weeks (n=7-11) p, Relative Gdf15 mRNA levels in adipose tissues from C57BL/6N mice fed chow diet or HFD for 12 weeks (left, n=3-6) and from ob/ob mice (right, n=5). q, Relative Gdf15 mRNA level in mature adipocytes and SVCs of eWAT from C57BL/6N mice fed chow diet or HFD for 12 weeks estimated by real-time RT-PCR (n=3) (left). Expression of Adipoq and Cd45 mRNA level showing successful fractionation of adipocytes and SVCs (middle and right). r, Relative Gdf15 mRNA level in macrophage and non-macrophage fractions of SVCs of eWAT estimated by real-time RT-PCR after MACS separation. All data are shown as means ± SEM. *P < 0.05, **P < 0.01 and ***P < 0.001 by two-tailed Student’s t-test (d,e,f,g,n,p) or one-way ANOVA with Tukey’s test (o,q,r). (n.s., not significant).
