Conserved Protein Domain Family
alphaCoV_Nsp14
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cd21660: alphaCoV_Nsp14 
nonstructural protein 14 of alphacoronavirus
Nonstructural protein 14 (Nsp14) of coronavirus (CoV) plays an important role in viral replication and transcription. It consists of 2 domains with different enzymatic activities: an N-terminal exoribonuclease (ExoN) domain and a C-terminal cap (guanine-N7) methyltransferase (N7-MTase) domain. ExoN is important for proofreading and therefore, the prevention of lethal mutations. The association of Nsp14 with Nsp10 stimulates its ExoN activity; the complex hydrolyzes double-stranded RNA in a 3' to 5' direction as well as a single mismatched nucleotide at the 3'-end mimicking an erroneous replication product. The Nsp10/Nsp14 complex may function in a replicative mismatch repair mechanism. N7-MTase functions in mRNA capping. Nsp14 can methylate GTP, dGTP as well as cap analogs GpppG, GpppA and m7GpppG. The accumulation of m7GTP or Nsp14 has been found to interfere with protein translation of cellular mRNAs.
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Statistics
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PSSM-Id: 394959
Aligned: 56 rows
Threshold Bit Score: 987.993
Created: 24-Apr-2020
Updated: 17-Oct-2022
Structure
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Aligned Rows:
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Feature 1:N7-MTase active site [active site]
Evidence:
  • Comment:based on the structure of SARS coronavirus Nsp14 with bound S-adenosyl-L-homocysteine and guanosine-P3-adenosine-5',5'-triphosphate
  • Comment:the (guanine-N7) methyltransferase (N7-MTase) domain functions in mRNA capping
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Sequence Alignment
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Feature 1                                                                                         
NP_839967       5 CGLFKDCSR.[1].DDLLPPSHANTFMSLADNFKTD.[1].YLAVQIGV.[5].YEHVISFMGFRFDINIP.[2].HTLF 73   Porcine epidemic d...
BBA21167     1457 CGLFKDCSK.[1].EHYIPPAYATTYISLSDSFKTS.[1].GLAVNVGT.[4].YASVISYMGFRFDVNIP.[2].HTLF 1524 Ferret coronavirus
AVY53334     5522 CGLFKDCAK.[1].EHYIPPAYATTYISLSDSFKTS.[1].GFAVDVGN.[4].YASVISYMGFRFDANIP.[2].HTLF 5589 Alphacoronavirus M...
ATP66783     5235 VGLFKDCYR.[1].VFTLNPAQSHTYEALSDKYKSG     NLAVWFGE.[4].YEHVISMLGFKLDQTVP.[2].HSLF 5301 Shrew coronavirus
ASF90469     5178 TGLFKSCVR.[1].PYEVGPAYATTFMHLTDRFKVS.[1].DLAVALPS.[4].FEDVVNLMGFKLDVVPD.[2].NPLF 5245 Wencheng Sm shrew ...
ATP66788     5566 SGLFKNCIR.[1].GHDLPPNHARSFLDLDSRFKVT.[1].ELAVHIGS.[4].YEHVLSWMGFRFDACPK.[2].HTMF 5633 Rodent coronavirus
YP_009256195 5471 CGLFKDCSK.[1].ERYIPPAYATTYISLSDSFKTS.[1].GLAVNVGT.[4].YASVISYMGFRFDVNIP.[2].HTMF 5538 Ferret coronavirus
YP_009380520 1507 SGLFKNCIR.[1].VHELPPNHARSFLDLNSKFKVN.[1].ELAVHIGV.[4].YEHVLSWMGFRFDACPE.[2].HTMF 1574 Coronavirus AcCoV-...
ASF90459     5164 TGLFKSCVR.[1].PYEVGPAYATTFMHLTDRFKVS.[1].DLAVALPS.[4].FEDVVNLMGFKLDVVPD.[2].NPLF 5231 Wencheng Sm shrew ...
YP_009019180 5531 CGLFKDCAK.[1].EHYIPPAYATTYISLSDSFKTN.[1].GFAVDVGN.[4].YASVISYMGFRFDANIP.[2].HTLF 5598 Mink coronavirus s...


Feature 1                                                                                         
NP_839967      74 CTRDFAMRNVRGWLGFDVEGAHVVGSNVGTNVPLQLGFSN.[1].VDFVVRPEGCVV.[3].GDYIKPVRARAPPGEQFA 147  Porcine epidemic d...
BBA21167     1525 CTRDFAMRNVRAWLGFDVEGAHVCGDNVGTNVPLQLGFSN.[1].VDFIVQPEGCVV.[3].GNDIRPVKSRAPPGEQFA 1598 Ferret coronavirus
AVY53334     5590 CTRDFAIRNVRAWLGFDVESAHVCGDNIGTNVPLQLGFSN.[1].VDFVVQPEGCVV.[3].GSSICPVKSRAPPGEQFA 5663 Alphacoronavirus M...
ATP66783     5302 CTRTFAIKHVRGWIGFDVEAAHVCGENIGTNVPLQLGFST.[1].IDFIAQPEGCVV.[3].GNQIIPVKARAPPGEQFT 5375 Shrew coronavirus
ASF90469     5246 CTRSYAIQHVRGWIGFDVESVHCCTDNVGTNLPLQLGFSN.[1].FNFVVKPEACVM.[3].KVYWKNVKTKVPPGEQFL 5319 Wencheng Sm shrew ...
ATP66788     5634 CTRDFAIRNVRGWLGFDVEGAHVCGDNCGTNVPLQLGFSN.[1].VDFVVQPEASYA.[3].GILVKNVKARAPPGEQFT 5707 Rodent coronavirus
YP_009256195 5539 CTRDFAMRNVRAWLGFDVEGAHVCGDNIGTNVPLQLGFSN.[1].VDFVVQPEGCVV.[3].GNGIVPVKSRAPPGEQFA 5612 Ferret coronavirus
YP_009380520 1575 CTRDFAIRNVRGWLGFDVEGAHVCGENCGTNVPLQLGFSN.[1].VDFVVQPEAGYA.[3].GILIKGVKARAPPGEQFT 1648 Coronavirus AcCoV-...
ASF90459     5232 CTKSYAIQHVRGWIGLDVESVHCCTDNVGTNLPLQIGFSN.[1].LNFVVKPEACVM.[3].KVYWKNVKTKVPPGEQFT 5305 Wencheng Sm shrew ...
YP_009019180 5599 CTRDFAIRNVRAWLGFDVESAHVCGDNIGTNVPLQLGFSN.[1].VDFVVQPEGCVV.[3].GSSICPVKSRAPPGEQFA 5672 Mink coronavirus s...


Feature 1                                                                                         
NP_839967     148 HLLPLLKRGQPWDVVRKRIVQMCSDYL.[2].LSDILIFVLWAGGLELTTMRYFVKIGPSKSCDCGKVATCYNS.[2].H 221  Porcine epidemic d...
BBA21167     1599 HLIPLMRKGQAWSIVRRRIVQMVCDYF.[2].LSDIVIFVLWAGGLELTTMRYFVKIGEQRKCECGIVAACYNS.[2].C 1672 Ferret coronavirus
AVY53334     5664 HLIPLMRRGQAWSIVRRRIVQMVCDYF.[2].LSDIVIFVLWAGGLELTTMRYFVKIGVPCKCDCGVVATCYNS.[2].G 5737 Alphacoronavirus M...
ATP66783     5376 HLIPLLRKGKPWSTVRRQIVQMCSDQL.[2].KSDIVIFVTWACGLELSTLRYFAKIGPHKTCFCGKTASCVCL.[2].N 5449 Shrew coronavirus
ASF90469     5320 HLIPLMRKTQDWNVVRKRIVSMLCDQF.[2].VTNEIIILTWAHGMELMTMRYFVKIGVVKRCYCGRRPKFYDS.[2].V 5393 Wencheng Sm shrew ...
ATP66788     5708 HLVPLLRKGQPWAVVRKRVIQMVCDHL.[2].LSDVVVFVLWAGGLELTTMRYFVKIGPSRNCHCGRVAQCYSS.[2].A 5781 Rodent coronavirus
YP_009256195 5613 HLIPLMRRGQAWSIVRRRIVQMVCDYF.[2].LSDIVIFVLWAGGLELTTMRYFVKIGEQRKCDCGIIAACYNS.[2].C 5686 Ferret coronavirus
YP_009380520 1649 HLVPLLRKGQPWVVVRKRIVQMVCDHL.[2].LSDVVVFVLWAGGLELTTMRYFVKIGPPRNCHCGRIAQCYSS.[2].S 1722 Coronavirus AcCoV-...
ASF90459     5306 HLIPLMRKTQDWSVVRKRIVSMLCDQF.[2].VTSEIIILTWAHGMELMTMRYFVKIGVVKRCYCGMEAKFYDS.[2].V 5379 Wencheng Sm shrew ...
YP_009019180 5673 HLIPLMRRGQAWSIVRRRIVQMVCDYF.[2].LSDVVIFVLWAGGLELTTMRYFVKIGVPCKCDCGVVATCYNS.[2].G 5746 Mink coronavirus s...


Feature 1                                                                                    #    
NP_839967     222 TYCCFKHA.[1].GCDYLYNPYCIDIQQWGYKGSLSLNHHEHCNVHRNEHVASGDAIMTRCLAIHDCF.[3].VDWSITY 295  Porcine epidemic d...
BBA21167     1673 TYHCMRHA.[1].GCDYLYNPFCIDIQQWGYTGSLSMNHHEICNVHKNEHVASGDAIMTRCLAIHDCF.[3].VDWSITY 1746 Ferret coronavirus
AVY53334     5738 TYHCMKHA.[1].GCDYLYNPYCIDIQQWGYTGSLSMNHHEVCNVHRNEHVASGDAIMTRCLAVHDCF.[3].VDWSITY 5811 Alphacoronavirus M...
ATP66783     5450 KYCCFDHS.[1].GCDYLYNPFVVDIQTWGYSGKLSVAHDEHCNLHRNAHIASGDAIMTHCLAAHDCF.[3].VNWDITY 5523 Shrew coronavirus
ASF90469     5394 EFKCLFHG.[1].GCDYLYNPKLVDVQQWGYVGKLADNHDMHCALHRNAHVASGDAIMTRCLAVYDSF.[3].VEWNIVY 5467 Wencheng Sm shrew ...
ATP66788     5782 AFSCLRHA.[1].GCDYLYNPYAFDIQQWGYTGSLSSNHHRYCNVHFNEHVASGDAIMTRCLAIYDCF.[3].VHWDITY 5855 Rodent coronavirus
YP_009256195 5687 TYRCMKHA.[1].GCDYLYNPFCIDIQQWGYTGSLSMNHHEICNVHKNEHVASGDAIMTRCLAIHDCF.[3].VDWSITY 5760 Ferret coronavirus
YP_009380520 1723 AFSCLRHA.[1].GCDYLYNPYAFDIQQWGYVGSLSANHHRYCNVHFNEHVASGDAIMTRCLAIYDCF.[3].VCWNITY 1796 Coronavirus AcCoV-...
ASF90459     5380 EFKCLFHG.[1].GCDYLYNPKLVDVQQWGYVGKLADNHDMYCALHRNAHVASGDAIMTRCLAVYECF.[3].VEWNIVY 5453 Wencheng Sm shrew ...
YP_009019180 5747 TYHCMKHA.[1].GCDYLYNPYCIDIQQWGYTGSLSMNHHEVCNVHRNEHVASGDATMTRCLAVHDCF.[3].VDWSITY 5820 Mink coronavirus s...


Feature 1                     #  ##  #                      #### #                ###            #
NP_839967     296 PFI.[2].EAVINKSGRIVQSHTMRSVLK.[2].NPKAIYDIGNPKGIRCAV.[1].DAKWFCFDKNP.[1].NSNVKTL 361  Porcine epidemic d...
BBA21167     1747 PFI.[2].EEAINKAGRVVQAHVVRAAIN.[2].NPSSIHDVGNPKGIRCVT.[1].SLPWFCYDKDP.[1].NSNVRCL 1812 Ferret coronavirus
AVY53334     5812 PFI.[2].EAAINKAGRVVQSHVVRAAIK.[2].NPSAIHDVGNPKGIRCVT.[1].PLPWFCYDKDP.[1].NSNVRCL 5877 Alphacoronavirus M...
ATP66783     5524 PFI.[2].EKRLNASLRVIQRNLMTAIIK.[2].GVKTVYDIGNPKGIKCSE.[1].DVVWKFFDKQP.[1].NDNVVVF 5589 Shrew coronavirus
ASF90469     5468 PYI.[2].EKAINAAGRFVERHIMSVFLK.[2].KPEAIHDIGNPKGIRCVV.[1].DKSWYCYDKDP.[1].NSNVREL 5533 Wencheng Sm shrew ...
ATP66788     5856 PYI.[2].EQDINAAGRYVERHLMNTFVK.[2].NPKAIYDVGNPKGIRICD.[1].KGSWYCYDKEP.[1].NNNVVKL 5921 Rodent coronavirus
YP_009256195 5761 PFI.[2].EESINKAGRVVQSHVVRAAIS.[2].NPSSIHDVGNPKGIRCVT.[1].SLPWFCYDKDP.[1].NSNVRCL 5826 Ferret coronavirus
YP_009380520 1797 PYI.[2].EKDINAAGRYVERHLMNSFLK.[2].NPKAIYDIGNPKGIRICD.[1].KGSWFCYDKEP.[1].NNNVVKL 1862 Coronavirus AcCoV-...
ASF90459     5454 PYI.[2].EKAINAAGRFVERHVMSVFLK.[2].KPEAVHDIGNPKGIRCVV.[1].DKSWYCYDKDP.[1].NSNVKEL 5519 Wencheng Sm shrew ...
YP_009019180 5821 PFI.[2].EATINKAGRVVQSHVVRAAIK.[2].NPSAIHDVGNPKGIRCVT.[1].PLPWFCYDKDP.[1].NSNVRCL 5886 Mink coronavirus s...


Feature 1         ##                   #####           #                     # ##  # #            
NP_839967     362 EYD     YITHGQFDGLCLFWNCNVDMYPEFSVVCRFDTRCRS.[2].NLEGCNGGSLYVNNHAFHTPAFDKRAFAKLK 433  Porcine epidemic d...
BBA21167     1813 EYD     FITHGQMPGLMLFWNCNVDMYPEQSIVCRFDTRTRS.[2].SLEGCNGGALYVNNHAFHTPAYDRRAFAKLK 1884 Ferret coronavirus
AVY53334     5878 DYD     FITHGQMPGLMLFWNCNVGMYPECSIVCRFDTRTRS.[2].SLEGCNGGALYVNNHAFHTEAYDRRAFAKLQ 5949 Alphacoronavirus M...
ATP66783     5590 SYD.[2].EHKSMFKDGLTMFWNCNVNVYPENAVVCRFDTRTRS.[2].SLLGPNGGCVYVNNHAFYTEPFDRRSFCHLQ 5663 Shrew coronavirus
ASF90469     5534 CYD     YETHGQFKGLMLFWNCNVSCYPEFSLVCRFDTRVCN.[2].NLEGVNGGSLYVNKHAFHTDFYDKRAFVNLK 5605 Wencheng Sm shrew ...
ATP66788     5922 EYD     YTVHGQFDGLCLFWNCNVDMYPGFSLVCRFDTKHRS.[2].SLEGVNGGCLYVNKHAFHTPAFDRRALAKLQ 5993 Rodent coronavirus
YP_009256195 5827 EYD     FITHGQMPGLMLFWNCNVDMYPEQSIVCRFDTRTRS.[2].SLEGCNGGALYVNNHAFHTPAYDRRAFAKLK 5898 Ferret coronavirus
YP_009380520 1863 EYD     YTVHGQFDGLCLFWNCNVDMYPGFSLVCRFDTKHRS.[2].SLEGVNGGCLYVNKHAFHTPAFDRRAFGKLQ 1934 Coronavirus AcCoV-...
ASF90459     5520 CYD     YETHGQFKGLMLFWNCNVNCYPEFSLVCRFDTRVCN.[2].NLEGVNGGSLYVNKHAFHTDFYDRRAFANLK 5591 Wencheng Sm shrew ...
YP_009019180 5887 DYD     FITHGQMPGLMLFWNCNVDMYPECSVVCRFDTRTRS.[2].SLEGCNGGALYVNNHAFHTEAYDRRAFAKLK 5958 Mink coronavirus s...


Feature 1                                                                     #                   
NP_839967     434 PMPFFFYDDTECD.[ 7].YVPLRASNCITKCNVGGAVCSKHCAMYHSYVNAYNTFTSAGFTIWVPTSFDTYNLWQTFSN 514  Porcine epidemic d...
BBA21167     1885 PMPFFYFDDSTCD.[ 7].YVPLKSNVCITKCNIGGAVCKKHAALYRKYVEEYNMFVQAGFNIWCPPNFDTYTLWHSFVN 1965 Ferret coronavirus
AVY53334     5950 PLPFFFFDDSTCE.[ 7].YVPLKSNVCITRCNIGGAVCKKHAALYRKYVEDYNTFVQAGFTIWGPTNFDIYTLWQSFVS 6030 Alphacoronavirus M...
ATP66783     5664 PMQFFFFDDTECE.[ 7].MVPLRSNTCFTKCNVGGAGCRKHVQMYKEYLECFNLAVEQGFTFWVPKTFDLYHLWALLRK 5744 Shrew coronavirus
ASF90469     5606 QIPFFFFDDSDCE.[ 7].YVPLSDKNCITRCNIGNAICNKHAKLYQEYLKKYNLFVSNGFNLWGPKSFDIYNLWSLLKG 5686 Wencheng Sm shrew ...
ATP66788     5994 ALPFFFFDDSDCD.[11].YVPLRSNVCVTKCNVGGAVCKKHASLYKTYVQKYNEFTQNGFTLWGPQNFDLFNLWQLVSK 6078 Rodent coronavirus
YP_009256195 5899 PMPFFYFDDSTCD.[ 7].YVPLKSNVCITKCNIGGAVCRKHAALYRKYVEDYNMFVQAGFNIWCPTNFDTYTLWHSFVN 5979 Ferret coronavirus
YP_009380520 1935 PLPFFFFDDGECD.[11].YVPLRSNVCITKCNVGGAVCKKHASLYKTYVQKYNDFTQNGFTLWGPRNFDLFNLWQLVSR 2019 Coronavirus AcCoV-...
ASF90459     5592 QIPFFFFDDSECE.[ 7].YVPLSDKNCITKCNIGNAICSKHAKLYQEYLEKYNLFVSNGFNLWGPKSFDVYNLWSLLRG 5672 Wencheng Sm shrew ...
YP_009019180 5959 PLPFFFFDDSTCE.[ 7].YVPLKSNVCITRCNIGGAVCKKHAALYRKYVEDYNTFVQAGFTIWGPSNFDTYTLWQGFVN 6039 Mink coronavirus s...

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