Use the NCBI Toolkit to compare genomic sequences
BLAST
Find regions of similarity between biological sequences with the Basic Local Alignment Search Tool (BLAST). There are many ways to BLAST:
- SmartBLAST: find proteins highly similar to your query
- Primer-BLAST: design primers specific to your PCR template
- Global Alignment: compare two sequences across their entire span
Try the BLAST experimental ClusteredNR database
Identify sequences of interest faster and more efficiently across a wider range of proteins, organisms, and evolutionary distances using our new ClusteredNR database. ClusteredNR is derived from the default BLASTP nr database with sequences clustered within 90% identity and 90% length to each other.
Use the NCBI Toolkit to visualize data
Explore genome data
Analyze annotated eukaryotic genome assemblies across the tree of life with NCBI’s web-based genome browser, Genome Data Viewer (GDV). You can add BLAST results, custom data, or content from Track Hubs to the display, and download slices of annotation data and high-quality graphics for publication directly from the browser.
Align sequences
Display and analyze multiple nucleotide or protein sequence alignments to highlight regions of sequence similarity and differences using the Multiple Sequence Alignment (MSA) Viewer. MSA Viewer's display is easily customizable to show only the alignments and metadata you need and is perfectly paired with BLAST- and COBALT-based analyses.
Compare genomic structures
Compare assembled genomes and easily identify genomic changes that may be significant to biology and evolution with CGR’s new Comparative Genome Viewer (CGV). View assembly-assembly comparisons from more than 50 annotated genomes at the whole genome, chromosome, or chromosome-region levels.
Need some help?
Contact us for customer service support
Get CGR updates
Join our mailing list to receive CGR updates and opportunities to provide feedback.