rap1 telomere-binding protein Rap1 [Schizosaccharomyces pombe (fission yeast)] - Gene

Summary

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Gene symbol: rap1
Gene description: telomere-binding protein Rap1
Locus tag: SPOM_SPBC1778.02
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Schizosaccharomyces pombe (strain: 972h-)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces
Also known as: lot2
Old locus tag: SPBC1778.02
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Genomic context

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See rap1 in Genome Data Viewer

Location:: chromosome: II

Exon count:: 3

Sequence:: Chromosome: II; NC_003423.3 (3098271..3100698)

Chromosome II - NC_003423.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003423.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The cooperative assembly of shelterin bridge provides a kinetic gateway that controls telomere length homeostasis.
Title: The cooperative assembly of shelterin bridge provides a kinetic gateway that controls telomere length homeostasis.
our data demonstrate that Rap1-Bqt4-dependent telomere association to nuclear envelope preserves telomere integrity in post-mitotic cells, preventing telomeric transcription and recombination
Title: Nuclear envelope attachment of telomeres limits TERRA and telomeric rearrangements in quiescent fission yeast cells.
We demonstrate that deletion of Rap1 is protective in this context, preventing the growth crisis that is otherwise triggered by degradation of telomeric R-loops in survivors with linear chromosomes. These findings suggest that upregulation of telomere-engaged TERRA, or altered recruitment of shelterin components, can support telomerase-independent telomere maintenance.
Title: RNA-DNA Hybrids Support Recombination-Based Telomere Maintenance in Fission Yeast.
Fission yeast cells lacking shelterin components Taz1 or Rap1 showed higher gross chromosomal rearrangements (GCRs) rates compared to wild-type, accumulating large chromosome deletions. Study results highlight unappreciated functions of the shelterin components Taz1 and Rap1 in maintaining genome stability, specifically by preventing non-telomeric GCRs.
Title: Telomere-binding proteins Taz1 and Rap1 regulate DSB repair and suppress gross chromosomal rearrangements in fission yeast.
Rap1 is phosphorylated by CK2 at multiple sites, which promotes interactions with Bqt4 and Poz1. Phosphorylation at Ser496 was found to be crucial for both Rap1-Bqt4 and Rap1-Poz1 interactions.These mechanisms mediate proper telomere tethering to the NE and the formation of the silenced chromatin structure at chromosome ends.
Title: Casein kinase 2 regulates telomere protein complex formation through Rap1 phosphorylation.
Telosome formation depends on the conserved telomeric proteins Taz1 and Rap1, and RNA. Rap1Delta telomeres lack detectable telosomes. Taz1 and Rap1 act together in organizing the telosome.
Title: Fission yeast telosomes: non-canonical histone-containing chromatin structures dependent on shelterin and RNA.
Whereas Rap1 hyper-phosphorylation observed in meiotic prophase does not have an apparent role in bouquet formation, the intrinsic negative charge of Rap1 is important for forming interactions with its binding partners.
Title: Telomere protein Rap1 is a charge resistant scaffolding protein in chromosomal bouquet formation.
fission yeast shelterin (shelterin subunits Poz1, Rap1 and Taz1 )maintains telomere length homeostasis by coordinating the differential arrival of leading (Polepsilon) and lagging (Polalpha) strand DNA polymerases at telomeres to modulate Rad3(ATR) association
Title: Fission yeast shelterin regulates DNA polymerases and Rad3(ATR) kinase to limit telomere extension.
Identification of the functional domains of the telomere protein Rap1 in Schizosaccharomyces pombe
Title: Identification of the functional domains of the telomere protein Rap1 in Schizosaccharomyces pombe.
it is proposed that the telomere dissociation from the nuclear envelope promoted by Rap1 phosphorylation is critical for the fidelity of chromosome segregation in closed mitosis
Title: Telomere-nuclear envelope dissociation promoted by Rap1 phosphorylation ensures faithful chromosome segregation.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by PomBase

Function	Evidence Code	Pubs
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables telomeric DNA binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in meiotic attachment of telomeric heterochromatin to spindle pole body	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic attachment of telomeric heterochromatin to spindle pole body	IPI Inferred from Physical Interaction more info	PubMed
involved_in mitotic telomere tethering at nuclear periphery	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protection from non-homologous end joining at telomere	IBA Inferred from Biological aspect of Ancestor more info
involved_in protection from non-homologous end joining at telomere	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in telomere capping	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in telomere maintenance	IGI Inferred from Genetic Interaction more info	PubMed
involved_in telomere maintenance via telomere lengthening	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
is_active_in chromosome, telomeric repeat region	IDA Inferred from Direct Assay more info	PubMed
is_active_in meiotic spindle pole body	IDA Inferred from Direct Assay more info	PubMed
part_of nuclear telomere cap complex	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	HDA more info	PubMed
is_active_in nucleus leading edge	IDA Inferred from Direct Assay more info	PubMed
part_of shelterin complex	IBA Inferred from Biological aspect of Ancestor more info
part_of shelterin complex	IPI Inferred from Physical Interaction more info	PubMed

General protein information

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Preferred Names: telomere-binding protein Rap1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003423.3 Reference assembly

Range

3098271..3100698

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001022207.3 → NP_596285.1 telomere-binding protein Rap1 [Schizosaccharomyces pombe]

See identical proteins and their annotated locations for NP_596285.1

Status: PROVISIONAL

UniProtKB/Swiss-Prot

Q96TL7, Q9UTW1, Q9UU15

Conserved Domains (4) summary

cd11655
Location:122 → 170

rap1_myb-like; DNA-binding modules of yeast Rap1 and related proteins

cd11670
Location:645 → 693

Sp_RAP1_RCT; C-terminal domain of S. pombe RAP1 protein

pfam09197
Location:494 → 603

Rap1-DNA-bind; Rap1, DNA-binding

pfam16589
Location:1 → 103

BRCT_2; BRCT domain, a BRCA1 C-terminus domain