Figure 2

(A) Nucleotide sequence of M13mp18 used for designing the inner and outer primers. The nucleotide sequence of the sense strand of M13mp18 DNA is shown. DNA sequences used for primer design are shown by heavy lines. Probe sequences used for Southern blot hybridization are indicated by dotted lines and the restriction sites for BamHI, PstI and PvuII are indicated by boxes. Numbers at the left end correspond to the positions in M13mp18 (GenBank accession no. {"type":"entrez-nucleotide","attrs":{"text":"X02513","term_id":"58253"}}X02513). (B) Schematic representation of the anticipated structure of the amplified product. B+, B–, F+ and F– in the first row are as in Figure 1B. The second row indicates the probes used for Southern blot hybridization. The restriction sites for PstI, PvuII and BamHI are shown as lines and the sizes of the restriction fragments are in the boxes.