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Table 1

Protein expression data for the Vip3Aa19 and APH4 proteins in fuzzy seeds [μg/g dry weight (dw) and μg/g fresh weight (fw)] from cotton COT102 (a)

Fuzzy seedsμg/g dry weight (dw)μg/g fresh weight (fw)
Vip3Aa192.16 (b)  ± 0.470 (c) 1.83 ± 0.468
(1.01–2.99) (d) (0.725–2.62)
APH4< LOQ (e) < LOQ (e)
Pollen
Vip3Aa19< LOD (f) < LOD (f)
APH4 (g) 10.2 ± 1.444.34 ± 0.787
(8.38–11.7)(3.69–5.48)
(a)Number of samples n = 20 in fuzzy seeds and n = 4 for in pollen.
(b)Mean.
(c)Standard deviation.
(d)Range.
(e)For APH4 in fuzzy seeds 12 samples were below the limit of detection (LOD = 0.125 μg/g dw) and 8 were below the limit of quantification (LOQ = 0.250 μg/g dw).
(f)For Vip3Aa19 all samples were below the limit of detection (LOD = 3.20 μg/g dw).
(g)The concentration of APH4 in pollen represents the sum of all iterations collected through sequential exhaustive extractions, therefore no further adjustment for extraction efficiency is applicable.
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