Figure 1

Bcl3 suppression reduces cell confluency over time. (A) Relative Bcl3 expression 6 days post-Bcl3 siRNA transfection in 4 breast cancer cell lines was assessed using qRT-PCR. (B) Cell titre blue was used to assess cell viability following 2 and 6 days of siRNA treatment, displayed as relative viability loss compared to that of scRNA controls. (C–F) IncuCyte analysis software (version 2022B) was used to analyse differences in cell confluency in (C) MCF-7, (D) ZR751, (E) T47D and (F) BT474 cells following Bcl3 suppression with data normalised to the confluency at the time of siRNA transfection. Error bars represent ± SEM of n = 3 minimum, with statistical differences determined using a 2-tailed t test; * p < 0.05. ** p < 0.01, and *** p < 0.001.