FIG. 7

Formation of native E1-E2 complexes after castanospermine (CST) or tunicamycin treatment, analyzed with the conformation-sensitive MAb H2. BHK-21 cells were coinfected with vTF7-3 and vHCV170-809 (V) or vTF7-3 and vpTM1 (M). Infected cells were incubated in the presence or absence of 1 mM CST or 5 μg of tunicamycin per ml, labeled for 2 h with [³⁵S]methionine in the presence of the same concentrations of drugs, and lysed with Triton X-100. Cell lysates were used for immunoprecipitation with a conformation-sensitive (H2) or -insensitive (A4) MAb and analyzed by SDS-PAGE (10% polyacrylamide). Agg, aggregates. The sizes (in kilodaltons) of molecular mass markers are indicated on the right.