Fig. 5

NACC1 mRNA level in response to overexpression of miR-218-5p, -124-3p, and −23b-3p. (A–F) Levels of miR-23b-3p, -124-3p and -218-5p in C-33A and CaSki cells transfected with each mimic. (G) NACC1 mRNA level in response to overexpression of miR-23b-3p, -124-3p, -218-5p or to the co-expression of the three microRNAs, compared to scrambled, in C-33A cells and (H) CaSki cells. The levels of miRNAs and NACC1 were normalized with RNU6 and GAPDH, respectively. Statistical analysis was performed using GraphPad Prism v.8.0. When the value was p < 0.05, differences were considered statistically significant. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.