Protection against Fatal Sindbis Virus Encephalitis by Beclin, a Novel Bcl-2-Interacting Protein

Xiao Huan Liang; Linda K. Kleeman; Hui Hui Jiang; Gerald Gordon; James E. Goldman; Gail Berry; Brian Herman; Beth Levine

doi:10.1128/jvi.72.11.8586-8596.1998

FIG. 4

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Confocal laser scanning microscopy of COS7 cells expressing Bcl-2 and Beclin. (A and B) Cell cotransfected with pSG5/bcl-2 and pSG5/flag-beclin and stained with anti-flag epitope (A) and anti-human Bcl-2 (B) antibodies. (D and E) Cell cotransfected with pSG5/bcl-2 and pSG5/flag-beclinDBcl-2BD and stained with anti-flag epitope (D) and anti-human Bcl-2 (E) antibodies. (C and F) Computerized overlays of panels A and B (C) and panels D and E (F); yellow color corresponds to overlap of FITC and rhodamine staining. Confocal slides were 1 μm thick.