Figure 5

Cellular association of different P(AA–OH-AAm)-6AF determined via flow cytometry measurements. Polymer concentration: 1 mM_RU, 50,000 cells seeded in 250 μL in a 48 well plate. Normalized MFI was calculated by eq 3. (A, B) Association of polymers with L929 mouse fibroblasts in DMEM supplemented with 10% FBS. Incubation for indicated time points at 37 °C. (C) Incubation at 37 or 4 °C in DMEM supplemented with 10% FBS for 4 h. Reduction was calculated by eq 4. Statistical analysis (one-way ANOVA with Tukey test) was performed for 4 h incubation time (A, B) to compare the association efficiency of the different P(AA–OH-AAm)-6AF. For temperature-dependent experiments (C), the statistical analysis compared the association at 37 °C versus 4 °C per polymer type. *Indicates a significance of p < 0.5. All other comparisons were not statistically significant at p < 0.5.