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FIG. 4

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Effect of incubation time on AAV5/βGal-mediated gene transfer to human airway epithelia. Human airway epithelia were exposed to 500 particles of AAV5/βGal per cell from the apical surface. The epithelia were then rinsed after 30, 60, 90, 360, and 720 min and were incubated for 2 weeks prior to analysis of β-galactosidase activity. Data are β-galactosidase activities per milligram of protein ± SEMs (n = 4). Asterisk indicates P < 0.01.

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