Figure 4

Overexpression of the GmMYB183 gene improved Al tolerance in Arabidopsis. (a) After germination in 1/2 MS medium, three-day-old seedlings were transferred to solid medium containing 0, 50, 100, and 150 μmol/L AlCl₃ (500 μmol/L CaCl₂, pH 4.5) for 7 days (scale bar = 1 cm). (b) Relative root growth after treatment with various AlCl₃ concentrations for 7 days. (c) Hematoxylin staining for roots treated with 50 μmol/L AlCl₃ (scale bar = 1 mm). (d) Citrate secretion of roots treated with various AlCl₃ concentrations for 24 h. OE: Overexpression of GmMYB183 in Arabidopsis; OE-m: Overexpression of GmMYB183-S36A in Arabidopsis. Columns and bars represent means ± standard errors (n = 3). Different letters represent significant differences (Duncan, p < 0.05).