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Biochem J. 1988 Jun 15; 252(3): 893–896.
doi: 10.1042/bj2520893
PMCID: PMC1149231
PMID: 3421930

Damage to DNA concurrent with lipid peroxidation in rat liver slices.

C G Fraga and A L Tappel
Author information Copyright and License information PMC Disclaimer

Abstract

Lipid peroxidation and DNA damage were evaluated in liver slices incubated for 2 h at 37 degrees C with 1 mM-t-butyl hydroperoxide (t-BOOH), 1 mM-BrCCl3 or 50 microM-ferrous iron. t-BOOH induced the greatest amount of damage to DNA and increased the production of thiobarbituric acid-reactive substances (TBARS). Both phenomena depended on the incubation time. Ferrous iron induced both DNA damage and TBARS production, and BrCCl3 did not induce significant DNA damage and was the weakest TBARS inducer. Butylated hydroxytoluene at 1 mM inhibited both DNA damage and TBARS production. DNA damage and lipid peroxidation in liver slices were correlated, indicating that these events were concurrent.

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Selected References

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