Figure 5

Expression of cell cycle-regulated genes in breast tumors. The tumor samples have been ordered by hierarchical clustering using 1753 genes as described previously (Perou et al., 2000). (A) Expression profiles are shown of 386 of the 1134 cell cycle-regulated clones that were also present in the breast cancer study (Perou et al., 2000). The clones are ordered by their similarity of gene expression profiles across both data sets by hierarchical clustering. (B) Detailed views of the clusters identified in Figure ​Figure5A.5A. A subset of genes is expressed at lower levels in the more proliferative tumors (NODE379X, cluster i; NODE303X, cluster iv) and includes genes that peak at M/G1, S, and G2 phases. Genes for core cell cycle processes that peak at G2 and M phases (NODE142X, ii) or at G1 and S phases (NODE133X, iii) are highly expressed in the more proliferative tumors; many of these genes are the same genes found in the breast tumor proliferation cluster. A subset of cell cycle regulated genes is expressed in a heterogeneous manner (NODE345X, v). An enhanced figure with all gene names is available as Supplemental Figure 11.