Figure 2

Manual versus automated gating templates. (A) Representative manual analysis of a CEF-stimulated sample from Experiment 4. Sequential gates on small lymphocytes, CD3⁺ cells, and CD3⁺CD8⁺. cells are applied and the percent CD69⁺IFNg⁺ cells are determined from a plot gated on all of these regions. (B) Dynamic gating template for the same data file as above. Sequential dynamic gates ("Snap-To" gates) are applied as above, except that negative populations are also gated so as to provide a boundary for the movement of the positive region. The percent CD69⁺IFNg⁺ cells obtained is very similar to that obtained by manual gating in this example, since manual gating was performed so as to include CD3^dim and CD8^dim cells.