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Genetics. 1996 Mar; 142(3): 749–759.
doi: 10.1093/genetics/142.3.749
PMCID: PMC1207016
PMID: 8849885

Mutations in the RNA Polymerase II Transcription Machinery Suppress the Hyperrecombination Mutant Hpr1Δ of Saccharomyces Cerevisiae

H. Y. Fan, K. K. Cheng, and H. L. Klein
Author information Copyright and License information PMC Disclaimer

Abstract

The soh1, soh2 and soh4 mutants were isolated as suppressors of the temperature-dependent growth of the hyperrecombination mutant hpr1 of Saccharomyces cerevisiae. Cloning and sequence analysis of these suppressor genes has unexpectedly shown them to code for components of the RNA polymerase II transcription complex. SOH2 is identical to RPB2, which encodes the second largest subunit of RNA polymerase II, and SOH4 is the same as SUA7, encoding the yeast transcription initiation factor TFIIB. SOH1 encodes a novel 14-kD protein with limited sequence similarity to RNA polymerases. Interestingly, SOH1 not only interacts with factors involved in DNA repair, but transcription as well. Thus, the Soh1 protein may serve to couple these two processes. The Soh1 protein interacts with a DNA repair protein, Rad5p, in a two-hybrid system assay. Soh1p may functionally interact with components of the RNA polymerase II complex as suggested from the synthetic lethality observed in soh1 rpb1Δ104, soh1 soh2-1 (rpb2), and soh1 soh4 (sua7) double mutants. Because mutations in SOH1, RPB2 and SUA7 suppress the hyperrecombination phenotype of hpr1 mutants, this suggests a link between recombination in direct repeats and transcription.

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Selected References

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  • Aguilera A, Klein HL. Genetic control of intrachromosomal recombination in Saccharomyces cerevisiae. I. Isolation and genetic characterization of hyper-recombination mutations. Genetics. 1988 Aug;119(4):779–790. [PMC free article] [PubMed] [Google Scholar]
  • Aguilera A, Klein HL. HPR1, a novel yeast gene that prevents intrachromosomal excision recombination, shows carboxy-terminal homology to the Saccharomyces cerevisiae TOP1 gene. Mol Cell Biol. 1990 Apr;10(4):1439–1451. [PMC free article] [PubMed] [Google Scholar]
  • Arndt KT, Styles CA, Fink GR. A suppressor of a HIS4 transcriptional defect encodes a protein with homology to the catalytic subunit of protein phosphatases. Cell. 1989 Feb 24;56(4):527–537. [PubMed] [Google Scholar]
  • Biggin MD, Gibson TJ, Hong GF. Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963–3965. [PMC free article] [PubMed] [Google Scholar]
  • Fan HY, Klein HL. Characterization of mutations that suppress the temperature-sensitive growth of the hpr1 delta mutant of Saccharomyces cerevisiae. Genetics. 1994 Aug;137(4):945–956. [PMC free article] [PubMed] [Google Scholar]
  • Feaver WJ, Svejstrup JQ, Bardwell L, Bardwell AJ, Buratowski S, Gulyas KD, Donahue TF, Friedberg EC, Kornberg RD. Dual roles of a multiprotein complex from S. cerevisiae in transcription and DNA repair. Cell. 1993 Dec 31;75(7):1379–1387. [PubMed] [Google Scholar]
  • Feinberg AP, Vogelstein B. "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum. Anal Biochem. 1984 Feb;137(1):266–267. [PubMed] [Google Scholar]
  • Guzder SN, Qiu H, Sommers CH, Sung P, Prakash L, Prakash S. DNA repair gene RAD3 of S. cerevisiae is essential for transcription by RNA polymerase II. Nature. 1994 Jan 6;367(6458):91–94. [PubMed] [Google Scholar]
  • Guzder SN, Sung P, Bailly V, Prakash L, Prakash S. RAD25 is a DNA helicase required for DNA repair and RNA polymerase II transcription. Nature. 1994 Jun 16;369(6481):578–581. [PubMed] [Google Scholar]
  • Gyuris J, Golemis E, Chertkov H, Brent R. Cdi1, a human G1 and S phase protein phosphatase that associates with Cdk2. Cell. 1993 Nov 19;75(4):791–803. [PubMed] [Google Scholar]
  • Hampsey M. A tester system for detecting each of the six base-pair substitutions in Saccharomyces cerevisiae by selecting for an essential cysteine in iso-1-cytochrome c. Genetics. 1991 May;128(1):59–67. [PMC free article] [PubMed] [Google Scholar]
  • Hekmatpanah DS, Young RA. Mutations in a conserved region of RNA polymerase II influence the accuracy of mRNA start site selection. Mol Cell Biol. 1991 Nov;11(11):5781–5791. [PMC free article] [PubMed] [Google Scholar]
  • Hill J, Donald KA, Griffiths DE, Donald G. DMSO-enhanced whole cell yeast transformation. Nucleic Acids Res. 1991 Oct 25;19(20):5791–5791. [PMC free article] [PubMed] [Google Scholar]
  • Hoffman CS, Winston F. A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli. Gene. 1987;57(2-3):267–272. [PubMed] [Google Scholar]
  • Ito H, Fukuda Y, Murata K, Kimura A. Transformation of intact yeast cells treated with alkali cations. J Bacteriol. 1983 Jan;153(1):163–168. [PMC free article] [PubMed] [Google Scholar]
  • Johnson RE, Henderson ST, Petes TD, Prakash S, Bankmann M, Prakash L. Saccharomyces cerevisiae RAD5-encoded DNA repair protein contains DNA helicase and zinc-binding sequence motifs and affects the stability of simple repetitive sequences in the genome. Mol Cell Biol. 1992 Sep;12(9):3807–3818. [PMC free article] [PubMed] [Google Scholar]
  • Johnson RE, Prakash S, Prakash L. Yeast DNA repair protein RAD5 that promotes instability of simple repetitive sequences is a DNA-dependent ATPase. J Biol Chem. 1994 Nov 11;269(45):28259–28262. [PubMed] [Google Scholar]
  • Klein HL. Genetic control of intrachromosomal recombination. Bioessays. 1995 Feb;17(2):147–159. [PubMed] [Google Scholar]
  • Laurent BC, Treitel MA, Carlson M. Functional interdependence of the yeast SNF2, SNF5, and SNF6 proteins in transcriptional activation. Proc Natl Acad Sci U S A. 1991 Apr 1;88(7):2687–2691. [PMC free article] [PubMed] [Google Scholar]
  • Nonet ML, Young RA. Intragenic and extragenic suppressors of mutations in the heptapeptide repeat domain of Saccharomyces cerevisiae RNA polymerase II. Genetics. 1989 Dec;123(4):715–724. [PMC free article] [PubMed] [Google Scholar]
  • Nonet M, Sweetser D, Young RA. Functional redundancy and structural polymorphism in the large subunit of RNA polymerase II. Cell. 1987 Sep 11;50(6):909–915. [PubMed] [Google Scholar]
  • Orr-Weaver TL, Szostak JW. Yeast recombination: the association between double-strand gap repair and crossing-over. Proc Natl Acad Sci U S A. 1983 Jul;80(14):4417–4421. [PMC free article] [PubMed] [Google Scholar]
  • Pinto I, Ware DE, Hampsey M. The yeast SUA7 gene encodes a homolog of human transcription factor TFIIB and is required for normal start site selection in vivo. Cell. 1992 Mar 6;68(5):977–988. [PubMed] [Google Scholar]
  • Scafe C, Chao D, Lopes J, Hirsch JP, Henry S, Young RA. RNA polymerase II C-terminal repeat influences response to transcriptional enhancer signals. Nature. 1990 Oct 4;347(6292):491–494. [PubMed] [Google Scholar]
  • Sung P, Higgins D, Prakash L, Prakash S. Mutation of lysine-48 to arginine in the yeast RAD3 protein abolishes its ATPase and DNA helicase activities but not the ability to bind ATP. EMBO J. 1988 Oct;7(10):3263–3269. [PMC free article] [PubMed] [Google Scholar]
  • Svejstrup JQ, Feaver WJ, LaPointe J, Kornberg RD. RNA polymerase transcription factor IIH holoenzyme from yeast. J Biol Chem. 1994 Nov 11;269(45):28044–28048. [PubMed] [Google Scholar]
  • Tabor S, Richardson CC. DNA sequence analysis with a modified bacteriophage T7 DNA polymerase. Proc Natl Acad Sci U S A. 1987 Jul;84(14):4767–4771. [PMC free article] [PubMed] [Google Scholar]
  • Thomas BJ, Rothstein R. The genetic control of direct-repeat recombination in Saccharomyces: the effect of rad52 and rad1 on mitotic recombination at GAL10, a transcriptionally regulated gene. Genetics. 1989 Dec;123(4):725–738. [PMC free article] [PubMed] [Google Scholar]
  • Thompson CM, Koleske AJ, Chao DM, Young RA. A multisubunit complex associated with the RNA polymerase II CTD and TATA-binding protein in yeast. Cell. 1993 Jul 2;73(7):1361–1375. [PubMed] [Google Scholar]
  • Voelkel-Meiman K, Roeder GS. A chromosome containing HOT1 preferentially receives information during mitotic interchromosomal gene conversion. Genetics. 1990 Mar;124(3):561–572. [PMC free article] [PubMed] [Google Scholar]
  • Zhu Y, Peterson CL, Christman MF. HPR1 encodes a global positive regulator of transcription in Saccharomyces cerevisiae. Mol Cell Biol. 1995 Mar;15(3):1698–1708. [PMC free article] [PubMed] [Google Scholar]
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