Fig 3.

Ang2 induces pericyte-independent EC shape changes and remodeling of the basal lamina. Control (A) and Ang2-treated (B) PM preparations were labeled with Hoechst 33258 for nuclei (blue) and stained with anti-VE-cadherin (green) and antismooth muscle α-actin antibodies (red). This shows that Ang2-induced shape changes occur in capillaries with and without pericytes. Images at ×630. Control (C) and Ang2-treated (D) PM preparations were labeled with Hoechst 33258 for nuclei (blue) and with the HUI77 antibody against a cryptic collagen epitope (red). The higher level of signal present in the dilated capillaries shown in D indicates that the basal lamina is remodeled in response to Ang2. Images at ×400.