Factor H Mutations in Hemolytic Uremic Syndrome Cluster in Exons 18–20, a Domain Important for Host Cell Recognition

PMC full text:

Am J Hum Genet. 2001 Feb; 68(2): 485–490.

Published online 2001 Jan 17. doi: 10.1086/318203

Table 1

FH Intronic Primers and PCR Conditions^[Note]

IntronicPrimer Position	Primer Sequence	Annealing Temperature(°C)	Magnesium Concentration(mM)
Leader Sequence	F: ggagtgcagtgagaattggg	53	2.5
	R: caacaatgtcaaaagccactc
1	F: ctccatagatatggggttagg	55	1.5
	R: cagagccagactccatctc
2a	F: ccttacattcaatctgtcttc	53	1.5
	R: ccatcatagttaaactttcagg
2b	F: gacactcagaatggcatcgag	55	1.5
	R: gatcaggctgcattcgtttttg
3	F: cctccaatcttatcctgaggatg	55	1.5
	R: gctgatattccttagaatgaacg
4	F: cctgatggaaacaacatttctg	45	2.5
	R: cataaattagcactctacttttg
5	F: gccattttgtattatgctaagg	55	1.5
	R: cttactttgtatatacaataagac
6	F: gagtgtttattacagtaaaatttc	53	3.0
	R: gtgctctcctttcttcgatc
7	F: ttagtaactttagttcgtcttcag	55	3.0
	R: ggtccattggtaaaacaaggtg
8	F: ggttttcagttacaaatgactc	53	1.5
	R: gaaattatatcagcccccac
9	F: gctttttcttcttagaattgggg	53	3.0
	R: caactatccttaacaattcctc
10	F: tctgatgcccctctgtatgacc	45	3.0
	R: cttatttcagcaattgtaagataag
11	F: gaagaaaatctttccatttttactg	43	3.5
	R: caaaatacaaaagttttgacaag
12	F: acttgtcaaaacttttgtattttg	55	1.5
	R: ggaaatgttgaggcttatctg
13	F: gattaagtcataattttaccatgc	55	1.5
	R: cacacatacctattacttttcc
14	F: gtgataatttatgaaacagttattg	45.6	3.5
	R: ctctcttctttacacgaagcac
15	F: caaattatactcactttaaaatccg	55	3.0
	R: cccctcactttgataacaagag
16	F: gtgatgtcatagtagctcctg	55	1.5
	R: ctagagtccctgtttactttc
17	F: cgctattttagaatccattacatg	55	1.5
	R: ggcctcccaaagtgctggg
18	F: gtaactgttatcagttgatttgc	60	1.5
	R: tagccctgctatactccccc
19	F: ttcttccaggactcatttctttc	54	1.5
	R: gtgagtattttgttacaaacagtg
20	F: tttattcaaatcaatatgatgtttc	53	2.5
	R: agttctgaataaaggtgtgcac

Note.— Conditions were as follows: 94°C for 4 min, then 30 cycles of 94°C for 1 min, annealing temperature for 1 min, and 72°C for 1 min.