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Published online 2002 Jun 17. doi: 10.1073/pnas.122061399
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Figure 6

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Reduction of H2O2 by human TrxR and the effect of ebselen and Trx. To cuvettes containing 50 mM Tris⋅Cl, 1 mM EDTA, pH 7.5, and 100 μM NADPH was added 0.5 mM H2O2 and 17 nM human TrxR (hTrxR) (●), 17 nM hTrxR plus 2 μM ebselen (▴), or 17 nM hTrxR plus 2 μM ebselen and 4.5 μM human Trx (hTrx) (■). The A340 was determined against a blank with 17 nM TrxR but without H2O2.

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