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Figure 5
Test of translational independence of the second cistron in the dicistronic reporter construct. Phosphorimages of SDS–PAGE gels containing proteins labeled with [35S]methionine during in vitro translation. Rabbit reticulocyte lysates were programmed with 20 ng/µl of dicistronic RNA transcribed in vitro by T7 polymerase as indicated. Rluc translation is controlled by either the EMCV IRES (A), or ΔEMCV (B), Fluc translation is controlled by the three indicated sequences. Results identical to these were obtained using 10 ng/µl of RNA in separate reactions.
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