Matrices with Compliance Comparable to that of Brain Tissue Select Neuronal over Glial Growth in Mixed Cortical Cultures

Penelope C. Georges; William J. Miller; David F. Meaney; Evelyn S. Sawyer; Paul A. Janmey

doi:10.1529/biophysj.105.073114

FIGURE 2

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Phase images of astrocytes on soft (A) and hard (B) LN-coated PA gels 2 days after plating on gels. Astrocytes on soft gels are smaller and less spread than on hard gels. Cultures of dissociated embryonic cortices at 2 days in vitro on soft (C) and hard (D) gels show insignificant differences between neurons on the two gels. Scale bars, 50 μm (A,B) and 25 μm (C,D).