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FIG. 7.

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Measurement of the leakage of K+ (a) and other cellular materials (b) from B. breve JCM 1192 upon exposure to CA for 15 min (a) and 3 h (b). Cells (A660 of ∼0.6) were incubated with various concentrations of CA in 150 mM sodium phosphate buffer (pH 6.5) containing 1 mM MgSO4, 1.0 U/ml horseradish peroxidase, and 10 mM glucose. The K+ concentration (a) and the UV absorbance (b) at 260 nm (•) and 280 nm (○) of the cell-free supernatant were determined spectroscopically.

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