Fig. 3.

A/RT virus-mediated retrotransposition events show structural hallmarks of LINE-1 retrotransposition. Two events derived from A/RT virus-infected Gli36 cells were characterized. The flanking genomic sequences were identified by using blat searches against the Human Genome Sequence (Assembly May 2004; http://genome.ucsc.edu). The cartoons show the relative positions of each insertion in chromosomal DNA and the sequences of the respective preintegration and postintegration sites. The blue letters in the postintegration sequences indicate the target site duplications. The vertical arrows and red lettering indicate the L1 endonuclease cleavage sites in the preintegration sequences. Both insertions analyzed (4-1A and 4-2D) show hallmarks of conventional target-primed reverse transcription-dependent retrotransposition (2, 19, 20).