Mediator protein mutations that selectively abolish activated transcription

Lawrence C. Myers; Claes M. Gustafsson; Kathleen C. Hayashibara; Patrick O. Brown; Roger D. Kornberg

doi:10.1073/pnas.96.1.67

Figure 2

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Immunobloting analysis of wild-type and mutant holoenzymes. Mono-Q fractions of wild-type, Δmed2, and Δpgd1 holoenzymes were subjected to immunoblot analysis by using antibodies directed against Mediator components Med2, Pgd1, Med4, and Med7 (8). The amounts of the Δmed2 and Δpgd1 holoenzymes loaded on the gel were approximately three times greater than the amount of wild-type holoenzyme, to demonstrate the absence of Med2 and Pgd1 subunits.