Figure 4

RNA blot analysis of HIS4 and GAL1 induction in wild-type and Δmed2 strains. (A) Wild-type (MG106) and Δmed2 (MG107) mutant cells transformed with pRS313 (HIS3) (22) were grown in synthetic minimal medium (16) supplemented with 0.2 mM inositol, 2.0 mM leucine, 0.5 mM isoleucine, 0.5 mM valine, 0.4 mM tryptophan, 0.25 mM arginine, 0.1 mM adenine, 0.2 mM lysine, and 0.2 mM uracil to OD₆₀₀ = 0.8. For starvation conditions, 3-aminotriazol (3-AT) was added to 100 mM, and the cultures were harvested 3 hr later. The RNA blot was hybridized to radioactively labeled probes for HIS4 and ACT1. (B) Wild-type (MG106) and Δmed2 (MG107) mutant cells were grown in yeast extract/peptone/raffinose medium overnight, washed with water and transferred to yeast extract/peptone/glucose (Glu) or yeast extract/peptone/galactose (Gal) media at a density of OD₆₀₀ = 0.15, followed by harvest at OD₆₀₀ = 0.6. The RNA blot was hybridized to radioactively labeled probes for GAL1 and DED1.